Abstract
Epithelia in the human airways, from the nasal aperture to the alveoli, are covered in a protective film of fluid containing a number of antimicrobial proteins. Defensins are single-chain, strongly cationic peptides and are one of the most extensively studied classes of antimicrobial peptides. Moxifloxacin (MXF) is a fluoroquinolone that acts against both Gram positive and Gram negative bacteria. In this study, we evaluated the effects of HBD2, MXF and the association MXF/HBD2 on some cytokines and on the ICAM-1 expression in LPS-stimulated A549 cells. Our results suggest that by lowering the epithelial cell-derived IL-1beta, IL-6, IL-8 and ICAM-1 expression, the MXF/HBD2 association interferes with the multifunctional cytokine network evolving during inflammatory processes of the respiratory tract; this anti-inflammatory potential could be of great value in the treatment of inflammatory disorders.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Aza Compounds / pharmacology*
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Cell Adhesion / drug effects
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Cell Line
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Cytokines / genetics
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Cytokines / metabolism
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism
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Fluoroquinolones
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Gene Expression / drug effects
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Humans
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Inflammation / drug therapy
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Intercellular Adhesion Molecule-1 / genetics
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Intercellular Adhesion Molecule-1 / metabolism
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Interleukin-1beta / genetics
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Interleukin-1beta / metabolism
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Interleukin-6 / genetics
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Interleukin-6 / metabolism
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Interleukin-8 / genetics
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Interleukin-8 / metabolism
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Lipopolysaccharides / metabolism
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Lipopolysaccharides / pharmacology
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Lung / cytology
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Moxifloxacin
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Neutrophils / drug effects
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Quinolines / pharmacology*
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
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beta-Defensins / pharmacology*
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Aza Compounds
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CXCL8 protein, human
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Cytokines
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DEFB4A protein, human
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Fluoroquinolones
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IL6 protein, human
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Interleukin-1beta
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Interleukin-6
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Interleukin-8
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Lipopolysaccharides
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Quinolines
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Tumor Necrosis Factor-alpha
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beta-Defensins
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Intercellular Adhesion Molecule-1
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Moxifloxacin