Abstract
Reduction of colony forming units by rifampicin-isoniazid therapy given 9-17 weeks post-infection was made more pronounced by immunotherapy with a vaccine made of fragmented Mycobacterium tuberculosis cells detoxified and liposomed (RUTI), given on weeks 17, 19 and 21 post-infection, in the murine model of tuberculosis in C57BL/6 and DBA/2 inbred strains. RUTI triggered a Th1/Th2 response, as demonstrated by the production of IgG1, IgG2a and IgG3 antibodies against a wide range of peptides. The histological analysis did not show neither eosinophilia nor necrosis, and granulomatous infiltration was only slightly increased in C57BL/6 mice when RUTI was administered intranasally.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Bacterial / blood
-
Antitubercular Agents / therapeutic use*
-
Colony Count, Microbial
-
Combined Modality Therapy
-
Disease Models, Animal
-
Female
-
Immunoglobulin G / blood
-
Immunotherapy
-
Interferon-gamma / analysis
-
Isoniazid / therapeutic use*
-
Lung / microbiology
-
Lung / pathology
-
Mice
-
Mice, Inbred C57BL
-
Mice, Inbred DBA
-
Mycobacterium tuberculosis / immunology*
-
Rifampin / therapeutic use*
-
Spleen / microbiology
-
Tuberculosis / drug therapy
-
Tuberculosis / therapy*
-
Tuberculosis Vaccines / therapeutic use*
-
Tumor Necrosis Factor-alpha / analysis
Substances
-
Antibodies, Bacterial
-
Antitubercular Agents
-
Immunoglobulin G
-
Tuberculosis Vaccines
-
Tumor Necrosis Factor-alpha
-
Interferon-gamma
-
Isoniazid
-
Rifampin