Abstract
Of the five PDE4D isoenzymes, only the PDE4D4 cAMP specific phosphodiesterase was able to bind to SH3 domains. Only PDE4D4 and PDE4A5, but not any other PDE4A, B, C and D isoforms expressed in rat brain, bound to src, lyn and fyn kinase SH3 domains. Purified PDE4D4 could bind to purified lyn SH3. PDE4D4 and PDE4A5 both exhibited selectivity for binding the SH3 domains of certain proteins. PDE4D4 did not bind to WW domains. We suggest that an important function of the unique N-terminal region of PDE4D4 may be to allow for association with certain SH3 domain-containing proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3',5'-Cyclic-AMP Phosphodiesterases / chemistry*
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Amino Acid Sequence
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Animals
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Brain / enzymology
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Carrier Proteins / genetics
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Cyclic AMP / pharmacology*
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Cyclic Nucleotide Phosphodiesterases, Type 3
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Cyclic Nucleotide Phosphodiesterases, Type 4
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Humans
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Isoenzymes / metabolism
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Maltose-Binding Proteins
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Molecular Sequence Data
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Protein Binding
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Rats
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Recombinant Fusion Proteins / metabolism
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src Homology Domains / genetics*
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src-Family Kinases / metabolism
Substances
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Carrier Proteins
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Isoenzymes
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Maltose-Binding Proteins
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Recombinant Fusion Proteins
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Cyclic AMP
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src-Family Kinases
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3',5'-Cyclic-AMP Phosphodiesterases
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Cyclic Nucleotide Phosphodiesterases, Type 3
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Cyclic Nucleotide Phosphodiesterases, Type 4
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PDE4A protein, human
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PDE4D protein, human