Chest
Original ResearchCopdOxidative DNA Damage and Somatic Mutations: A Link to the Molecular Pathogenesis of Chronic Inflammatory Airway Diseases
Section snippets
Subjects
Informed consent was obtained from all subjects, and the protocol was approved by the medical ethics committee of the University Hospital of Heraklion, Crete, Greece (approval number 11980/20/10/2009). A total of 97 subjects were studied: 36 patients with COPD, 36 patients with bronchiectasis (non-COPD), 15 smokers without COPD, and 10 healthy subjects (nonsmokers). Patients were allocated into four groups: the first group consisted of 36 patients with GOLD (Global Initiative for Chronic
Results
COPD and bronchiectasis groups of patients were matched for age and smoking exposure but differed statistically in the parameters of lung function (FEV1 [% predicted], FVC [% predicted], and FEV1/FVC). Patients with ronchiectasis, smokers without COPD, and healthy subjects had normal lung function; thus, they were statistically significant different from patients with COPD (Table 1).
Discussion
We report higher levels of oxidant-induced DNA damage, as revealed by 8-OHdG marker, and increased frequency of acquired somatic mutations, as revealed by MSI, in patients with COPD when compared with smokers without COPD, patients with bronchiectasis, and healthy subjects. Our results could provide another link in the molecular pathogenesis of COPD, with the 8-OHdG levels reflecting the ongoing oxidative DNA damage and the MSI reflecting the inefficient DNA repair capacity of the organism.
To
Acknowledgments
Author contributions: Drs Tzortzaki, Dimakou, Neofytou, and Siafakas had full access to all the data and take responsibility for the integrity of the data and the accuracy of the data analysis.
Dr Tzortzaki: contributed to the study concept and design, analyzed and interpreted the data, drafted the manuscript, critically revised the manuscript for important intellectual content, and contributed to the statistical analysis.
Dr Dimakou: contributed to the study concept and design, acquired the
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Cited by (0)
Drs Dimakou and Neofytou contributed equally to this study.
Funding/Support: The authors have reported to CHEST that no funding was received for this study.
Reproduction of this article is prohibited without written permission from the American College of Chest Physicians (http://www.chestpubs.org/site/misc/reprints.xhtml).