Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Cloning and expression of a cDNA encoding an endothelin receptor

Nature volume 348, pages 730–732 (1990)Cite this article

Abstract

ENDOTHELINS are a newly described peptide family consisting of three peptides (ET-1, ET-2 and ET-3) which are the most potent vasoconstrictive peptides known1–3. They are crucial in the regulation of vascular smooth muscle tone1–3. The diverse functions of endothelins are thought to be mediated by interaction with many different receptors coupled to the inositol phosphate/calcium ion messenger pathway. However, because of the structural resemblance of the three peptides, the presence and nature of multiple endothelin receptors remain to be elucidated. We report here the cloning of a complementary DNA encoding a bovine endothelin receptor, which has a transmembrane topology similar to that of other G protein-coupled receptors and shows specific binding, with the highest selectivity to ET-1 in animal cells trans-fected with the cloned cDNA. This receptor messenger RNA is widely distributed in the central nervous system and peripheral tissues, particularly in the heart and lung. Our results support the view that there are other receptor subtypes.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Yanagisawa, M. & Masaki, T. Trends pharmacol. Sci. 10, 374–378 (1989).

    Article  CAS  Google Scholar 

  2. Yanagisawa, M. et al. Nature 332, 411–415 (1988).

    Article  ADS  CAS  Google Scholar 

  3. Takuwa, N., Takuwa, Y., Yanagisawa, M., Yamashita, K. & Masaki, T. J. biol. Chem. 264, 7856–7861 (1989).

    CAS  PubMed  Google Scholar 

  4. Masu, Y. et al. Nature 329, 836–838 (1987).

    Article  ADS  CAS  Google Scholar 

  5. Yokota, Y. et al. J. biol. Chem. 264, 17649–17652 (1989).

    CAS  Google Scholar 

  6. Shigemoto, R., Yokota, Y., Tsuchida, K. & Nakanishi, S. J. biol. Chem. 265, 623–628 (1990).

    CAS  Google Scholar 

  7. Tanaka, K., Masu, M. & Nakanishi, S. Neuron 4, 847–854 (1990).

    Article  CAS  Google Scholar 

  8. Lory, P., Richard, S., Rassendren, F. A., Tiaho, F. & Nargeot, J. FEBS Lett. 258, 289–292 (1989).

    Article  CAS  Google Scholar 

  9. Kozak, M. Nucleic Acids Res. 15, 8125–8148 (1987).

    Article  CAS  Google Scholar 

  10. O'Dowd, B. F., Lefkowitz, R. J. & Caron, M. G. A. Rev. Neurosci. 12, 67–83 (1989).

    Article  CAS  Google Scholar 

  11. Dixon, R. A. F., Strader, C. D. & Sigal, I. S. Annual Reports in Medicinal Chemistry Volume 23, 221–233 (Academic, New York, 1988).

    Google Scholar 

  12. McFarland, K. C. et al. Science 245, 494–499 (1989).

    Article  ADS  CAS  Google Scholar 

  13. Segaloff, D. FASEB J. 4, A1851 (1990).

    Google Scholar 

  14. Hubbard, S. C. & Ivatt, R. J. A. Rev. Biochem. 50, 555–583 (1981).

    Article  CAS  Google Scholar 

  15. O'Dowd, B. F., Hnatowich, M., Caron, M. G., Lofkowitz, R. J. & Bouvier, M. J. biol. Chem. 264, 7564–7569 (1989).

    CAS  Google Scholar 

  16. Kemp, B. E. & Pearson, R. B. Trends Biochem. Sci. 15, 342–346 (1990).

    Article  CAS  Google Scholar 

  17. Kishimoto, A. et al. J. biol. Chem. 260, 12492–12499 (1985).

    CAS  Google Scholar 

  18. Préat, T. et al. Nature 347, 87–89 (1990).

    Article  ADS  Google Scholar 

  19. Watanabe, H. et al. Biochem. biophys. Res. Commun. 161, 1252–1259 (1989).

    Article  CAS  Google Scholar 

  20. Emori, T., Hirata, Y., & Marumo, F. FEBS Lett. 263, 261–264 (1990).

    Article  CAS  Google Scholar 

  21. Koseki, C., Imai, M., Hirata, Y., Yanagisawa, M. & Masaki, T. Am. J. Physiol. 256, R858–R866 (1989).

    Article  CAS  Google Scholar 

  22. Nakanishi, S. et al. Recent Prog. Hormone Res. 46, 59–84 (1990).

    CAS  Google Scholar 

  23. Birnstiel, M. L., Busslinger, M. & Strub, K. Cell 41, 349–359 (1985).

    Article  CAS  Google Scholar 

  24. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. USA. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  25. Wilkes, B. M. et al. Am. J. Physiol. 258, E864–E870 (1990).

    CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Shigetada Nakanishi: To whom correspondence should be addressed.

Authors and Affiliations

  1. Institute for Immunology, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Hiroshi Arai, Seiji Hori, Ichiro Aramori, Hiroaki Ohkubo & Shigetada Nakanishi

Authors
  1. Hiroshi Arai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seiji Hori
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ichiro Aramori
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroaki Ohkubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shigetada Nakanishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arai, H., Hori, S., Aramori, I. et al. Cloning and expression of a cDNA encoding an endothelin receptor. Nature 348, 730–732 (1990). https://doi.org/10.1038/348730a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/348730a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing