Cell Stem Cell
Volume 7, Issue 1, 2 July 2010, Pages 127-133
Journal home page for Cell Stem Cell

Short Article
Primary Tumor Genotype Is an Important Determinant in Identification of Lung Cancer Propagating Cells

https://doi.org/10.1016/j.stem.2010.05.021Get rights and content
Under an Elsevier user license
open archive

Summary

Successful cancer therapy requires the elimination or incapacitation of all tumor cells capable of regenerating a tumor. Therapeutic advances therefore necessitate the characterization of the cells that are able to propagate a tumor in vivo. We show an important link between tumor genotype and isolation of tumor-propagating cells (TPCs). Three mouse models of the most common form of human lung cancer each had TPCs with a unique cell-surface phenotype. The cell-surface marker Sca1 did not enrich for TPCs in tumors initiated with oncogenic Kras, and only Sca1-negative cells propagated EGFR mutant tumors. In contrast, Sca1-positive cells were enriched for tumor-propagating activity in Kras tumors with p53 deficiency. Primary tumors that differ in genotype at just one locus can therefore have tumor-propagating cell populations with distinct markers. Our studies show that the genotype of tumor samples must be considered in studies to identify, characterize, and target tumor-propagating cells.

Highlights

► Identification of the first lung tumor-propagating cell population ► Lung cancers of different genotype have tumor-propagating cells with distinct markers ► Tumor samples should be separated by genotype to study tumor-propagating cells

STEMCELL

Cited by (0)