Asthma and lower airway diseaseBeneficial effects of high dose of L-arginine on airway hyperresponsiveness and airway inflammation in a murine model of asthma
Section snippets
Animals
Male BALB/c mice (8-10 weeks old; National Institute of Nutrition, Hyderabad, India) were acclimatized for a week before starting the experiments. All animals were maintained according to Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines, and protocols were approved by the Institutional Animal Ethics Committee.
Grouping of mice
In pilot experiments, mice were divided into 5 groups, and each (n = 6) was named according to sensitization/challenge/treatment: SHAM/PBS/vehicle
High-dose exogenous L-ARG reduces AHR and airway inflammation
To determine the possible beneficial effects of exogenous L-ARG supplementation in asthma, the effects of 25, 250, and 500 mg/kg L-ARG on Penh and AI were investigated. In dose titration experiments, allergic control mice (OVA/OVA/VEH) developed not only increased Penh (Fig 2, A) but also a dense infiltration of inflammatory cells, predominantly eosinophils and mononuclear cells, in perivascular and peribronchial regions compared with sham control mice (SHAM/PBS/VEH; Fig 2, B and C). Although
Discussion
L-arginine is a conditionally essential basic amino acid, and human beings normally consume 3.5 to 5 g daily.21 It has multiple pharmacologic properties (antioxidant, anti-inflammatory, antihypercholesterolemic, muscle relaxant, immunomodulatory, and so forth).12 These widespread activities could be a result of its effect on various metabolizing enzymes and their final metabolites.22 The use of L-ARG has been reported for various disease conditions (angina pectoris, congestive heart failure,
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Supported by projects NWP0033 and MLP 5501 at the Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research, Government of India. U.M. and G.D.L. are the recipients of fellowships from the Indian Council of Medical Research.
Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest.