Fast track — ArticlesMicroscopic-observation drug susceptibility and thin layer agar assays for the detection of drug resistant tuberculosis: a systematic review and meta-analysis
Introduction
The growing problem of drug resistance in Mycobacterium tuberculosis is accompanied by increasing demand for quick, cheap, and easy techniques to detect resistance.1 Patients infected with multidrug resistant (MDR) or extensively drug resistant (XDR) strains of M tuberculosis need treatment regimens that include second-line drugs in addition to extended treatment duration.2 Even when treated appropriately, MDR and XDR tuberculosis have substantially worse outcomes3 and longer infectious periods than does drug-susceptible tuberculosis.4 Therefore, prevention of the spread of MDR and XDR tuberculosis is important, for which timely identification of such cases is the first and most crucial step.
Several methods to detect drug resistance are available, but none clearly satisfies the demands of quick, cheap, and easy. Traditional agar-based methods can take months for results. Commercial drug susceptibility testing with liquid culture decreases turnaround times but requires expensive equipment. Molecular detection of gene mutations associated with drug resistance has also been developed, with variable sensitivity reported especially for in-house methods.5 Commercial versions of line-probe assays have high accuracy6, 7 and were recently endorsed by WHO for rapid screening of MDR tuberculosis.8 However, the expertise needed for a laboratory to offer molecular diagnostics is too great for these tests to be implemented in many resource-constrained settings with poor laboratory infrastructure.
Although liquid cultures and line-probe assays have been endorsed by WHO and phased implementation is underway in many countries, interim measures are needed to meet the needs of low-income settings with high rates of MDR and XDR tuberculosis. Non-commercial techniques to test drug susceptibility with inexpensive and widely available laboratory equipment and supplies—such as microscopic-observation drug susceptibilty (MODS) and thin layer agar (TLA) assays—could be implemented in these settings with minimum cost and training. In both MODS and TLA testing, drug-free and drug-containing media (liquid for MODS, solid for TLA) are inoculated with specimens from patients, and cultures are microscopically examined for early growth or microcolonies.9, 10 Growth of M tuberculosis in or on drug-free media indicates a positive culture, whereas growth of M tuberculosis in or on both drug-free and drug-containing media indicates resistance.
We did a systematic review of published reports and a meta-analysis of studies examining the diagnostic accuracy, performance (including contamination rate and turnaround time), and outcomes important to patients of MODS and TLA assays for the detection of drug resistance in M tuberculosis. We followed a standard protocol for systematic reviews and meta-analyses,11 and used methods recommended by the Cochrane Diagnostic Test Accuracy Working Group.12
Section snippets
Search strategy and study selection
We searched PubMed, Embase, and Biosis for reports published between January, 1990, and February, 2009 (inclusive). The first search used the terms tuberculosis[mesh] OR mycobacter*[ti] OR acid-fast[ti] OR tuberculous[ti] and the second search used the terms MODS[tw] OR “microscopic-observation”[tiab] OR “drug-susceptibility”[ti] OR (drug[ti] AND susceptibility[ti]) OR microcolony[tiab] OR (liquid[ti] AND culture[ti]) OR thin-layer[tiab] OR (thin[tiab] AND layer[tiab] AND agar[tiab]) OR
Results
We identified 2072 citations, of which 1406 unique articles remained after exclusion of duplicate articles, and 54 remained after screening of titles and abstracts (figure 1). These articles were retrieved for full-text review, of which 12 were eligible for inclusion in the primary analysis: nine of MODS testing21, 22, 23, 24, 25, 26, 27, 28, 29 and three of the TLA assay30, 31, 32 (table 1; see webappendix for primary accuracy data from included studies). We identified two additional studies
Discussion
From pooled estimates of nine studies, MODS testing had high accuracy for detection of rifampicin resistance, but showed slightly lower sensitivity for detection of isoniazid resistance. We identified only three studies assessing the TLA assay; however, in all studies, the assay had 100% concordance with the reference standards for detection of rifampicin or isoniazid resistance.
For detection of isoniazid restistance with the MODS assay, studies in which a cutoff of 0·1 μg/mL was used had
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