Original articleRepopulation of a human alveolar matrix by adult rat type II pneumocytes in vitro: A novel system for type II pneumocyte culture☆
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2019, Progress in Biophysics and Molecular BiologyCitation Excerpt :The realization that the development of the lung was dependent on cell-cell communication was consistent with Grobstein's earlier discovery that the process of lung development was mediated by low molecular weight soluble growth factors Grobstein (1953). Moreover, when epithelial cells of the lung (Lwebuga-Mukasa et al., 1986) or liver (Michalopoulos et al., 1979) were cultured in isolation they lost their differentiated structure and function unless supplied with factors from their normal physiologic cellular environment, providing important clues to the fundamental nature of their development, homeostasis and repair (Warburton et al., 2010). In the ensuing decades, the paracrine regulation of many tissues and organs has been determined, beginning with the cross talk between the animal and vegetal poles of the zygote (Gurdon et al., 1997).
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2017, Current Research in Translational MedicineCitation Excerpt :Residual DNA is infrequently retained within the lung matrix with the majority of decellularization protocols reporting no detectable genomic DNA using various ultrasensitive methods [74,80]. Rodent studies indicate that the acellular lung scaffold is biomimetic, capable of supporting growth and development of primary fetal and adult lung-specific cell types including alveolar type II cells (AEC) [81] and vascular cells [82], or the differentiation of induced pluripotent stem cells or bone marrow-derived mesenchymal stromal cells (BMSC) introduced via the cannulated pulmonary artery or trachea. Reseeding is a complex operation and may only be achieved with the use of specialized perfusion culture systems which support widespread cell attachment, proliferation and retention and, importantly, the conditioning of the regenerated organ prior to transplantation [83].
Biocompatibility evaluation of tissue-engineered decellularized scaffolds for biomedical application
2016, Materials Science and Engineering CCitation Excerpt :Therefore, tissue engineering seems to be as a key technology to close gaps in the management of major tissue defects in reconstructive surgery and could obviate many of the risks and problems associated with donor organs, and at lower cost [2]. The first trials to prepare organ-specific extracellular matrix (ECM) were reported in the 1970s and 1980s using livers [3], lungs [4], kidneys and blood vessels [5]. Decellularization techniques were designed to remove cells and debris from tissues and organs (Fig. 1) while preserving the biochemical composition, biological activity, three-dimensional organization and integrity of the extracellular matrix, indicating the maintenance of native ECM-contained cues necessary for cells [6,7].
Advances in pulmonary therapy and drug development: Lung tissue engineering to lung-on-a-chip
2016, Biotechnology AdvancesCitation Excerpt :Lung tissue has sophisticated matrix to provide its main function, gas exchange or mechanical properties and growth of endothelial, epithelial and mesenchymal cells (Ott et al., 2010; Petersen et al., 2010). Therefore, to obtain these criteria and overcome difficulties of scaffold preparation, the process of recellularization of acellular natural tissue scaffolds is used (Lwebuga-Mukasa et al., 1986). This method is based on decellularization of the native lung as a suitable bio-scaffold.
Rational engineering of lung alveolar epithelium
2023, npj Regenerative Medicine
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Supported by Clinical Investigator Award NHLB KO8-HL-00784, the American Lung Association Grant-in-Aid to JL-M, NHLB RO1HLB-28373 to J. A. M., and an American Lung Association Training Fellowship to D. H. I.