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ICAM-1, ICAM-2 and ICAM-3 in the Sera of Patients with Idiopathic Pulmonary Fibrosis

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Abstract

In order to test the serum levels of ICAM-1, ICAM-2 and ICAM-3 in patients with idiopathic pulmonary fibrosis (IPF), twenty patients with IPF and eleven with secondary interstitial fibrosis (SIF), as well as forty healthy volunteers (HV) were studied. Serum intracellular adhesion molecules (ICAM) 1, 2 and 3 were assessed by ELISA. Functional respiratory tests, which included spirometry and lung diffusing capacity were simultaneously performed. Median values of serum ICAM-1 and ICAM-2 were higher in the patients' than in the healthy volunteers' (HV) group: IPF group: 946.60 ng/ml and 400.14 ng/ml; SIF group: 901.58 ng/ml and 378.27 ng/ml; HV group: 308.40 ng/ml and 217.55 ng/ml, respectively (p < 0.05). ICAM-3 serum levels were equal between the three groups. ICAM-2 negatively correlated to DLCO values. (p < 0.005).

It can be concluded that ICAM 1 and 2 are elevated in the sera of patients with pulmonary fibrosis. ICAM-2 might be associated with a more impaired clinical status.

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References

  1. Selman, M., and A. Pardo. 2002. Idiopathic pulmonary fibrosis: an epithelial/ fibroblastic crosss- talk disorder. Respir. Res. 3:3.

    Article  PubMed  Google Scholar 

  2. Gross, T. J., and G. W. Hunninghake. 2001. Idiopathic pulmonary fibrosis. NEJM 345:517–525.

    Article  PubMed  Google Scholar 

  3. Palmer, R. M., J. A. Stapleton, J. Sutherland, P. Y. Coward, R. F. Wilson, and D. A. Scott. 2002. Effect of nicotine replacement and quitting smoking on circulating adhesion molecule profiles (sICAM-1, sCD44v5, sCD44v6). Eur. J. Clin. Invest. 32:852–857.

    Article  PubMed  Google Scholar 

  4. Lehman, J. C., D. Jablonski- Westrich, U. Haubold, J. C. Gutierrez-Ramos, T. Springer, and A. Hamann. 2003. Overlapping and selective roles of endothelial intercellular adhesion molecule-1 (ICAM-1) and ICAM-2 in lymphocyte trafficking. J. Immunol. 171:2588–2593.

    PubMed  Google Scholar 

  5. van Kooyk, Y., and T. B. Geijtenbeek. 2002. A novel adhesion pathway that regulates dendritic cell trafficking and T cell interactions. Immunol. Rev. 186:47–56.

    Article  PubMed  Google Scholar 

  6. Hayashi, S., K. Abe, H. Matsuoka, S. Goya, H. Morishita, M. Mori, T. Arai, H. Kida, K. Nishino, Y. Takeda, T. Osaki, I. Tachibana, K. Kimura, S. Yokota, Y. Inoue, and M. Sakatani. 2004. Increased Level of Soluble E-Selectin in the Serum from Patients with Idiopathic Pulmonary Fibrosis. Inflammation 28:1–5.

    Article  PubMed  Google Scholar 

  7. Xaubet, A., C. Agusti, P. Luburich, J. Roca, C. Monton, M. C. Ayuso, J. A. Barbera, and R. Rodriguez-Roisin. 1998. Pulmonary function tests and CT Scan in the management of idiopathic pulmonary fibrosis. Am. J. Respir. Crit. Care. Med. 158:431–436.

    PubMed  Google Scholar 

  8. Idiopathic Pulmonary Fibrosis: Diagnosis and Treatment (International Consensus Statement). 2000. Am. J. Crit. Care. Med. 161:646–664.

  9. Copeland, S., J. Siddiqui, and D. Remick. 2004. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods. 284:99–106.

    Article  PubMed  Google Scholar 

  10. Roca, J., J. Sanchis, A. Agusti-Vidal, F. Segarra, D. Navajas, and R. Rodriguez-Roisin. 1986. Spirometric reference values from a mediterranean population. Bull. Eur. Physiopathol. Respir. 22:217–224.

    PubMed  Google Scholar 

  11. Roca, J., R. Rodriguez-Roisin, E. Cobo, F. Burgos, J. Perez, and J. L. Clausen. 1990. Single breath carbon monoxide diffusing capacity (DLco) prediction equations from a Mediterranean population. Am. Rev. Respir. Dis. 140:1026–1032.

    Google Scholar 

  12. Snedecor, G. W., and W. G. Cochran. 1978. Statistical Methods 6th ed. Amos: Iowa State University Press 294.

    Google Scholar 

  13. van Tits, L. J., F. de Waart, H. L. Hak-Lemmers, P. van Heijst, J. de Graaf, P. N. Demacker, and A. F. Stalenhoef. 2001. Effects of alpha-tocopherol on superoxide production and plasma intercellular adhesion molecule-1 and antibodies to oxidized LDL in chronic smokers. Free. Radic. Biol. Med. 30:1122–1129.

    Article  PubMed  Google Scholar 

  14. Wakelkamp, I. M., M. N. Gerding, J. W. van der Meer, M. F. Prummel, and W. M. Wiersinga. 2002. Smoking and disease severity are independent determinants of serum adhesion molecule levels in Graves' ophthalmopathy. Clin. Exp. Immunol. 127:316–320.

    Article  PubMed  Google Scholar 

  15. Vainer, B., and O. H. Nielsen. 2000. Changed colonic profile of P-selectin, platelet-endothelial cell adhesion molecule-1 (PECAM-1), intercellular adhesion molecule-1 (ICAM-1), ICAM-2, and ICAM-3 in inflammatory bowel disease. Clin. Exp. Immunol. 121:242–247.

    Article  PubMed  Google Scholar 

  16. Keaney, J. F. Jr, J. M. Massaro, M. G. Larson, R. S. Vasan, P. W. Wilson, I. Lipinska, D. Corey, P. Sutherland, J. A. Vita, and E. J. Benjamin. 2004. Heritability and correlates of intercellular adhesion molecule-1 in the Framingham Offspring Study. J. Am. Coll. Cardiol. 44:168–173.

    Article  PubMed  Google Scholar 

  17. Kalra, V. K., Y. Ying, K. Deemer, R. Natarajan, J. L. Nadler, and T. D. Coates. 1994. Mechanism of cigarette smoke condensate induced adhesion of human monocytes to cultured endothelial cells. J. Cell. Physiol. 160:154–162.

    Article  PubMed  Google Scholar 

  18. Witte, D. R., W. M. Broekmans, A. F. Kardinaal, I. A. Klopping-Ketelaars, G. van Poppel, M. L. Bots, C. Kluft, and J. M. Princen. 2003. Soluble intercellular adhesion molecule 1 and flow-mediated dilatation are related to the estimated risk of coronary heart disease independently from each other. Atherosclerosis 170:147–153.

    Article  PubMed  Google Scholar 

  19. Beeh, K. M., J. Beier, O. Kornmann, and R. Buhl. 2003. Neutrophilic inflammation in induced sputum of patients with idiopathic pulmonary fibrosis. Sarcoidosis Vasc. Diffuse Lung Dis. 20:138–143.

    PubMed  Google Scholar 

  20. Rusznak, C., P. R. Mills, J. L. Devalia, R. J. Sapsford, R. J. Davies, and S. Lozewicz. 2000. Effect of cigarette smoke on the permeability and IL-1beta and sICAM-1 release from cultured human bronchial epithelial cells of never-smokers, smokers, and patients with chronic obstructive pulmonary disease. Am. J. Respir. Cell. Mol. Biol. 23:530–536.

    PubMed  Google Scholar 

  21. Ashitani, J., H. Mukae, H. Ihiboshi, T. Hiratsuka, H. Taniguchi, H. Mashimoto, and S. Matsukura. 1997. Serum soluble adhesion molecules in patients with idiopathic pulmonary fibrosis and acute respiratory distress syndrome. Nihon Kyobu Shikkan gakkai Zasshi (abstract only) 35:942–947.

    Google Scholar 

  22. Ihn, I., S. Sato, M. Fujimoto, K. Takehara, and K. Tamaki. 1998. Increased serum levels of soluble vascular cell adhesion molecule-1 and e- selectin in patients with systemic sclerosis. Br. J. of Rheumatology 37:1188–1192.

    Article  Google Scholar 

  23. Shijubo, N., K. Imai, K. Shigehara, Y. Honda, H. Koba, M. Tsujisaki, L. HIno, A. Yachi, M. Ohmochi, and Y. Hiraga. 1994. Soluble intercellular adhesion molecule – 1 (ICAM- 1) in sera and bronchoalveolar lavage fluid of patients with idiopathic pulmonary fibrosis and sarcoidosis. Clin. Exp. Immunol. 95:156–161

    PubMed  Google Scholar 

  24. Shijubo, N., K. Imai, S. Aoki, M. Hirasawa, H. Sugawara, P. Koba, M. Tsujisaki, T. Sugiyama, Y. Hinoda, and A. Yachi. 1992. Circulating intercellular adhesion mollecule-1 (ICAM- 1) antigen in sera of patients with idiopathic pulmonary fibrosis. Clin. Exp. Immunol. 89:58–62.

    PubMed  Google Scholar 

  25. Nakao, A., Y. Hasegawa, Y. Tsuchiya, and K. Shimokata. 1995. Expression of Cell Adhesion Molecules in the Lungs of Patients With Idiopathic Pulmonary Fibrosis. Chest. 108:233–239.

    PubMed  Google Scholar 

  26. van Dinther-Janssen, A. C., T. C. van Maarsseveen, H. Eckert, W. Newman, and C. Meijer. 1993. Identical expression of ELAM- 1, VCAM- 1 and ICAM- 1 in sarcoidosis and usual interstitial pneumonitis. J. Pathol. 170:157–164.

    Article  PubMed  Google Scholar 

  27. Hamaguch, Y., Y. Nishizawa, M. Yasui, M. Hasegawa, Y. Kaburagi, K. Komura, T. Nagaoka, E. Saito, Y. Shimada, K. Takehara, T. Kadono, D. A. Stebber, T. F. Tedder, and S. Sato. 2002. Intercellular adhesion molecule-1 and L- selectin regulate bleomycin- induced lung fibrosis. Am. J. of Pathology. 161:1607–1618.

    Google Scholar 

  28. Bleijs, D. A., R. de Waal-Malefyt, C. G. Figdor, Y. van Kooyk. 1999. Co-stimulation of T cells results in distinct IL-10 and TNF-alpha cytokine profiles dependent on binding to ICAM-1, ICAM-2 or ICAM-3. Eur. J. Immunol. 29:2248–2258.

    Article  PubMed  Google Scholar 

  29. Kotovuori, A., T. Pessa-Morikawa, P. Kotovuori, P. Nortamo, and C. G. Gahmberg. 1999. ICAM-2 and a peptide from its binding domain are efficient activators of leukocyte adhesion and integrin affinity. J. Immunol. 162:6613–6620.

    PubMed  Google Scholar 

  30. Vainer, B., O. H. Nielsen, and T. Horn. 2000. Comparative studies of the colonic in situ expression of intercellular adhesion molecules (ICAM-1, -2, and -3), beta2 integrins (LFA-1, Mac-1, and p 150,95), and PECAM-1 in ulcerative colitis and Crohn's disease. Am. J. Surg. Pathol. 24:1115–1124.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Pulmonary Department, University of Thessaly, Greece

    Pelagia G. Tsoutsou, Konstantinos I. Gourgoulianis & Maria Mpaka

  2. Department of Microbiology, University of Thessaly, Greece

    Efthymia Petinaki, Smaragda Efremidou & Antonios Maniatis

  3. Physiology Department, University of Thessaly, Greece

    Pashalis Adam Molyvdas

  4. Nikis 33, Syntagma Athens, 105 57, Greece

    Pelagia G. Tsoutsou

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  1. Pelagia G. Tsoutsou
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  2. Konstantinos I. Gourgoulianis
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Correspondence to Pelagia G. Tsoutsou.

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Tsoutsou, P.G., Gourgoulianis, K.I., Petinaki, E. et al. ICAM-1, ICAM-2 and ICAM-3 in the Sera of Patients with Idiopathic Pulmonary Fibrosis. Inflammation 28, 359–364 (2004). https://doi.org/10.1007/s10753-004-6647-6

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