Skip to main content
Log in

Effects of Aging and Anti-Aging Caloric Restrictions on Carbonyl and Heat Shock Protein Levels and Expression

  • Research Article
  • Published:
Biogerontology Aims and scope Submit manuscript

Abstract

Heat shock proteins (Hsps) are induced by stressful stimuli and have been shown to protect cells and organs from such stresses both in vitro and in vivo, and play a positive role in lifespan determination. An attenuated response to stress is characteristic of senescence and no Hsp induction is observed upon exposure to stress and no protective effect of a mild stress is observed in cells from aged individuals. The artificial over-expression of Hsps, can produce a protective effect against a variety of damaging stimuli in cells from aged rats or aged humans, in whom cardiovascular disease is a major cause of morbidity in older age. Here, we show that aging significantly decreases the levels of Hsp27, Hsp60, Hsp72 and Hsc70 in right atrium and left ventricle of the rat heart, both at level of protein and of mRNA. Two different caloric restriction regimens have been found to counteract in part the decrease in the levels of Hsp expression in the aged heart tissue as well as the tendency to an increase of the levels of carbonyl in cardiac proteins. Our data suggest that cardiac Hsp levels may be a determinant of longevity in rodents, and that generation of new regimens of caloric restriction may eventually show how to improve modulation of cardiac aging.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • C Arnaud M Joyeux-Faure S Bottari D Godin-Ribuot C Ribuot (2004) ArticleTitleNew insight into the signalling pathways of heat stress-induced myocardial preconditioning: protein kinase Cepsilon translocation and heat shock protein 27 phosphorylation Clin Exp Pharmacol Physiol 31 129–133 Occurrence Handle10.1111/j.1440-1681.2004.03966.x Occurrence Handle1:CAS:528:DC%2BD2cXitlWntbo%3D Occurrence Handle15008954

    Article  CAS  PubMed  Google Scholar 

  • E Bergamini G Cavallini A Donati Z Gori (2003) ArticleTitleThe anti-ageing effects of caloric restriction may involve stimulation of macroautophagy and lysosomal degradation, and can be intensified pharmacologically Biomed Pharmacother 57 IssueID5–6 203–208 Occurrence Handle1:CAS:528:DC%2BD3sXlvVSlt70%3D Occurrence Handle12888255

    CAS  PubMed  Google Scholar 

  • G Cavallini A Clerico M Chicca ParticleDel Z Gori E Bergamini (1995) ArticleTitleEffects of different types of anti-aging dietary restrictions on age-related atrial natriuretic factor changes: an immunochemical and ultrastructural study Aging (Milano) 7 117–122 Occurrence Handle1:STN:280:BymD2cvptVA%3D

    CAS  Google Scholar 

  • P Csermely (2001) ArticleTitleChaperone overload is a possible contributor to ‘civilization diseases’ Trends Genet 17 701–704 Occurrence Handle10.1016/S0168-9525(01)02495-7 Occurrence Handle1:CAS:528:DC%2BD3MXosFKmtb4%3D Occurrence Handle11718923

    Article  CAS  PubMed  Google Scholar 

  • AM Cuervo JF Dice (2000) ArticleTitleAge-related decline in chaperone-mediated autophagy J Biol Chem 275 31505–31513 Occurrence Handle1:CAS:528:DC%2BD3cXntlCitLY%3D Occurrence Handle10806201

    CAS  PubMed  Google Scholar 

  • R Dahiya A Lin ME Bakircioglu ST Huang TF Lue (1997) ArticleTitlemRNA and protein expression of nitric oxide synthase and adrenoceptor alpha 1 in young and old rat penile tissues Br J Urol 80 300–306 Occurrence Handle1:CAS:528:DyaK2sXls1ymsr4%3D Occurrence Handle9284206

    CAS  PubMed  Google Scholar 

  • A Del Roso V Tata ParticleDe Z Gori E Bergamini (1991) ArticleTitleTransmural differences of lipofuscin pigment accumulation in the left ventricule of rat heart during growth and aging Aging 3 19–23 Occurrence Handle2065122

    PubMed  Google Scholar 

  • HA Demirel KL Hamilton RA Shanely N Tumer MJ Koroly SK Powers (2003) ArticleTitleAge and attenuation of exercise-induced myocardial HSP72 accumulation Am J Physiol Heart Circ Physiol 285 H1609–H1615 Occurrence Handle1:CAS:528:DC%2BD3sXosVKjtL0%3D Occurrence Handle12791594

    CAS  PubMed  Google Scholar 

  • MJ Forster BH RS Sohal (2000) ArticleTitleReversible effects of long-term caloric restriction on protein oxidative damage J Gerontol A Biol Sci Med Sci Nov 55 IssueID11 B522–B529 Occurrence Handle1:STN:280:DC%2BD3M%2Fjs1ynsQ%3D%3D

    CAS  Google Scholar 

  • D Giannessi C Caselli RL Vitale A Crucean B Murzi S Ry ParticleDel V Vanini A Biagini (2003) ArticleTitleA possible cardioprotective effect of heat shock proteins during cardiac surgery in pediatric patients Pharm Res 48 519–529 Occurrence Handle10.1016/S1043-6618(03)00193-2 Occurrence Handle1:CAS:528:DC%2BD3sXntVGksbo%3D

    Article  CAS  Google Scholar 

  • S Goto R Takahashi S Araki H Nakamoto (2002) ArticleTitleDietary restriction initiated in late adulthood can reverse age-related alterations of protein and protein metabolism Ann NY Acad Sci 959 50–56 Occurrence Handle1:CAS:528:DC%2BD38XktFWqtbw%3D Occurrence Handle11976185

    CAS  PubMed  Google Scholar 

  • F Hartl (1996) ArticleTitleMolecular chaperones in cellular protein folding Nature 381 571–579 Occurrence Handle10.1038/381571a0 Occurrence Handle1:CAS:528:DyaK28XjsFyltb8%3D Occurrence Handle8637592

    Article  CAS  PubMed  Google Scholar 

  • AR Heydari R Takahashi A Gutsmann et al. (1994) ArticleTitleHsp70 and aging Experientia 50 1092–1098 Occurrence Handle10.1007/BF01923466 Occurrence Handle1:CAS:528:DyaK2MXivFeguro%3D Occurrence Handle7988669

    Article  CAS  PubMed  Google Scholar 

  • AR Heydari B Wu R Takahashi et al. (1993) ArticleTitleExpression of heat shock protein 70 is altered by age and diet at the level of transcription Mol Cell Biol 13 2909–2918 Occurrence Handle1:CAS:528:DyaK3sXisFahsrc%3D Occurrence Handle7682654

    CAS  PubMed  Google Scholar 

  • LE Hightower (1991) ArticleTitleHeat shock stress proteins chaperones and proteotox-icity Cell 166 191–197

    Google Scholar 

  • E.G. Lakatta S.J. Sollow (2002) ArticleTitlePerspectives on mammalian cardiovascular aging:humans to molecules Comp cBiochem Physiol Part A 132 699–721

    Google Scholar 

  • EJ Lesnefsky CL Hoppel (2003) ArticleTitleIschemia-reperfusion injury in the aged heart: role of mitochondria Arch Biochem Biophys 420 287–297 Occurrence Handle10.1016/j.abb.2003.09.046 Occurrence Handle1:CAS:528:DC%2BD3sXptl2qsb0%3D Occurrence Handle14654068

    Article  CAS  PubMed  Google Scholar 

  • M Locke RM Tanguay (1996) ArticleTitleDiminished heat shock response in the aged myocardium Cell Stress Chaperones 1 251–260 Occurrence Handle1:CAS:528:DyaK2sXlvVOqtA%3D%3D Occurrence Handle9222610

    CAS  PubMed  Google Scholar 

  • AJL Macario E Convay Macario Particlede (2002) ArticleTitleSick chaperones and ageing: a perspective Ageing Res Rev 1 295–311 Occurrence Handle1:CAS:528:DC%2BD38XosFSmtbs%3D Occurrence Handle12039444

    CAS  PubMed  Google Scholar 

  • S Makrides (1983) ArticleTitleProtein synthesis and degradation during aging and senescence Bio Rev 83 393–422

    Google Scholar 

  • EJ Masoro (1999) Challenges of Biological Aging Springer Publishing Company New York

    Google Scholar 

  • EJ Masoro (2002) Caloric Restriction: A Key to Understand and Modulating Aging Elsevier Science Amsterdam

    Google Scholar 

  • K Merker A Stolzing T Grune (2001) ArticleTitleProteolysis, caloric restriction and aging Mech Ageing Dev 122 595–615 Occurrence Handle10.1016/S0047-6374(01)00219-6 Occurrence Handle1:CAS:528:DC%2BD3MXivFeisLg%3D Occurrence Handle11322989

    Article  CAS  PubMed  Google Scholar 

  • R Pamplona M Portero-Otin J Requena R Gredilla G Barja (2002) ArticleTitleOxidative, glycoxidative and lipoxidative damage to rat heart mitochondrial proteins is lower after 4 months of caloric restriction than in age-matched controls Mech Ageing Dev 123 1437–1446 Occurrence Handle10.1016/S0047-6374(02)00076-3 Occurrence Handle1:CAS:528:DC%2BD38XosFeqsrg%3D Occurrence Handle12425950

    Article  CAS  PubMed  Google Scholar 

  • P Rafiee Y Shi KA Pritchard SuffixJr H Ogawa AL Eis RA Komorowski CM Fitzpatrick JS Tweddell SB Litwin K Mussatto RD Jaquiss JE Baker (2003) ArticleTitleCellular redistribution of inducible Hsp70 protein in the human and rabbitheart in response to the stress of chronic hypoxia: role of protein kinases J Biol Chem 278 43636–43644 Occurrence Handle10.1074/jbc.M212993200 Occurrence Handle1:CAS:528:DC%2BD3sXosFSrsbY%3D Occurrence Handle12937165

    Article  CAS  PubMed  Google Scholar 

  • SIS Rattan (1995) ArticleTitleAgeing – a biological perspective Mol Aspects Med 16 439–508 Occurrence Handle1:STN:280:BymA2MbksVE%3D Occurrence Handle8748909

    CAS  PubMed  Google Scholar 

  • SIS Rattan (1996) ArticleTitleSynthesis, modifications, and turnover of proteins during aging Gerontol Exp 31 33–47 Occurrence Handle1:CAS:528:DyaK28Xitlans7o%3D

    CAS  Google Scholar 

  • AZ Reznick L Paker (1994) Methods Enzymol 33 357–363

    Google Scholar 

  • A Sanz R Gredilla R Pamplona M Portero-Otin E Vara JA Tresguerres G Barja (2005) ArticleTitleEffect of insulin and growth hormone on rat heart and liver oxidative stress in control and caloric restricted animals Biogerontology 6 15–26 Occurrence Handle10.1007/s10522-004-7380-0 Occurrence Handle1:CAS:528:DC%2BD2MXjtlKmt74%3D Occurrence Handle15834660

    Article  CAS  PubMed  Google Scholar 

  • C Selman R Gredilla S Phaneuf S Kendaiah G Barja C Leeuwenburgh (2003) ArticleTitleShort-term caloric restriction and regulatory proteins of apoptosis in heart skeletal muscle and kidney of Fischer 344 rats Biogerontology 4 141–147 Occurrence Handle10.1023/A:1024149923693 Occurrence Handle1:CAS:528:DC%2BD3sXksV2jtLk%3D Occurrence Handle12815313

    Article  CAS  PubMed  Google Scholar 

  • I Simonetti V Tata ParticleDe A Del Roso Z Gori E Bergamini (1990) ArticleTitleChanges in the transmural distribution of antioxidant enzyme activities across the left ventricle heart wall from rats fed ad libitum or food-restricted during growth and aging Arch Gerontol Geriatr 10 163–171 Occurrence Handle10.1016/0167-4943(90)90016-Y Occurrence Handle1:CAS:528:DyaK3cXkslOjtL4%3D Occurrence Handle15374511

    Article  CAS  PubMed  Google Scholar 

  • C Söti P Csermely (2000) ArticleTitleMolecular chaperones and the ageing process Biogerontology 1 225–233 Occurrence Handle11707899

    PubMed  Google Scholar 

  • JA Stuart B Karahalil BA Hogue NC Souza-Pinto VA Bohr (2004) ArticleTitleMitochondrial and nuclear DNA base excision repair are affected differently by caloric restriction FASEB J 18 595–597 Occurrence Handle1:CAS:528:DC%2BD2cXisV2itbY%3D Occurrence Handle14734635

    CAS  PubMed  Google Scholar 

  • GE Taffet TT Pham CJ Hartley (1997) ArticleTitleThe age-associated alterations in late diastolic function in mice are improved by caloric restriction J Gerontol A Biol Sci Med Sci 52 B285–B290 Occurrence Handle1:STN:280:DyaK1c%2Fms1KgsQ%3D%3D Occurrence Handle9402929

    CAS  PubMed  Google Scholar 

  • S Vittorini C Paradiso A Donati G Cavallini M Masini Z Gori M Pollera E Bergamini (1999) ArticleTitleThe age-related accumulation of protein carbonyl in rat liver correlates with the age-related decline in liver proteolytic activities J Gerontol A Biol Sci Med Sci 54 B318–B323 Occurrence Handle1:STN:280:DyaK1MvisVymtA%3D%3D Occurrence Handle10496537

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to D. Giannessi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Colotti, C., Cavallini, G., Vitale, R.L. et al. Effects of Aging and Anti-Aging Caloric Restrictions on Carbonyl and Heat Shock Protein Levels and Expression. Biogerontology 6, 397–406 (2005). https://doi.org/10.1007/s10522-005-4906-z

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10522-005-4906-z

Keywords

Navigation