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Differing distributions of CXCR3- and CCR4-positive cells among types of interstitial pneumonia associated with collagen vascular diseases

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Abstract

Interstitial pneumonia (IP) is an important complication in collagen vascular diseases (CVDs). We examined the distribution of helper T cell subsets in lung biopsies of cases of IP associated with CVD (CVD-IP). The tissues from 27 CVD-IP patients with rheumatoid arthritis (RA), 8 with polymyositis or dermatomyositis (PM/DM), and 8 with systemic sclerosis (SSc) were compared with those from 10 patients with idiopathic pulmonary fibrosis (IPF) in our previous study. The expressions of CXCR3 and CCR4 (chemokine receptors associated in vitro with Th1 and Th2 cells, respectively) in the mononuclear infiltrate were analyzed immunohistochemically. The positive cells were semiquantified in fibrosing areas of the CVD-IP and IPF cases. The number of CXCR3-positive cells was significantly greater in RA-IP than in PM/DM-IP, SSc-IP, or IPF, whereas there were fewer CCR4-positive cells in RA-IP, PM/DM-IP, and SSc-IP than in IPF. The CXCR3-/CCR4-positive cells ratio was significantly higher in RA-IP and PM/DM-IP (but not in SSc-IP) than in IPF. These results support previous reports of the dominance of Th2 cells in some SSc-IP and IPF cases. However, Th1-type immune responses may predominate in RA-IP and PM/DM-IP. Our findings suggest that the pathogenesis of CVD-IPs differs with the helper T cell subset.

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Acknowledgements

We thank Kyowa Hakko, Tokyo, Japan, and Dr. Masamichi Koike for supplying the CCR4 antibody. We also thank Mr. Seino Nagaya, Department of Pathology, Nagoya City University Medical School, for his excellent technical assistance.

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Authors and Affiliations

  1. Department of Internal Medicine and Molecular Science, Nagoya City University Medical School, Kawasumi 1, Mizyho-ku, Nagoya, Aichi, 467-8601, Japan

    Shigeki Shimizu, Takashi Niimi, Hiroyoshi Maeda, Shigeki Sato & Ryuzo Ueda

  2. Department of Pathology, Nagoya City University Medical School, Nagoya, Aichi, Japan

    Shigeki Shimizu & Tadaaki Eimoto

  3. Department of Internal Medicine, Toyokawa City Hospital, Toyokawa, Aichi, Japan

    Takeo Yoshinouchi

  4. Department of Respiratory Medicine, Nagoya Central Hospital, Nagoya, Aichi, Japan

    Takashi Niimi

  5. Division of Pathology, Matsuyama-shimin Hospital, Matsuyama, Japan

    Yuji Ohtsuki

  6. Department of Medicine and Therapeutics, Faculty of Medicine, University of the Ryukyus, Okinawa, 903-0215, Japan

    Jiro Fujita

  7. Department of Pathology, National Okayama Medical Center, Okayama, Japan

    Ichiro Yamadori

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  1. Shigeki Shimizu
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  2. Takeo Yoshinouchi
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Correspondence to Shigeki Sato.

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Shimizu, S., Yoshinouchi, T., Niimi, T. et al. Differing distributions of CXCR3- and CCR4-positive cells among types of interstitial pneumonia associated with collagen vascular diseases. Virchows Arch 450, 51–58 (2007). https://doi.org/10.1007/s00428-006-0330-2

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  • DOI: https://doi.org/10.1007/s00428-006-0330-2

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