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Jet exhaust particles alter human dendritic cell maturation

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Abstract

Objective and design

Among combustion-derived air pollutants, little is known about jet kerosene characteristics and effects.

Materials and methods

Particles yielded by experimental kerosene combustion in a jet engine were characterized with electron microscopy and X-ray energy dispersive spectroscopy. Immature human monocyte-derived dendritic cells were exposed for 18 h to 10, 25 or 100 μg/mL jet exhaust particles and/or Escherichia coli-derived endotoxin. Antigen-presenting and costimulation molecules (HLA DR, CD40, CD80, CD86, CD11c), tumor necrosis factor-α and interleukin-10 production were measured.

Results

The primary particles of jet exhaust are spherical (9.9 nm), carbonaceous and exert an adjuvant effect on human monocyte-derived dendritic cell maturation in vitro. Concomitant particle and endotoxin stimulation induced a high cytokine production with low antigen-presenting molecules; particle contact prior to endotoxin contact led to an opposite phenotype. Finally, low cytokine production and high costimulation molecules were present when particle adjunction followed endotoxin contact.

Conclusions

Jet exhaust particles act as adjuvants to endotoxin-induced dendritic cell maturation, suggesting possible implications for human health and a role for the time pattern of infectious and pollutant interplay.

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References

  1. Alexis NE, Lay JC, Zeman K, Bennett WE, Peden DB, Soukup JM, et al. Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy donors. J Allergy Clin Immunol. 2006;117:1396–403.

    Article  CAS  PubMed  Google Scholar 

  2. Andersen ZJ, Loft S, Ketzel M, Stage M, Scheike T, Hermansen MN, et al. Ambient air pollution triggers wheezing symptoms in infants. Thorax. 2008;63:710–6.

    Article  CAS  PubMed  Google Scholar 

  3. Downs SH, Schindler C, Liu LJ, Keidel D, Bayer-Oglesby L, Brutsche MH, et al. Reduced exposure to PM10 and attenuated age-related decline in lung function. New Engl J Med. 2007;357:2338–47.

    Article  CAS  PubMed  Google Scholar 

  4. McCreanor J, Cullinan P, Nieuwenhuijsen MJ, Stewart-Evans J, Malliarou E, Jarup L, et al. Respiratory effects of exposure to Diesel traffic in persons with asthma. New Engl J Med. 2007;357:2348–58.

    Article  CAS  PubMed  Google Scholar 

  5. Lippmann M. Health effects of airborne particulate matter. New Engl J Med. 2007;357:2395–6.

    Article  CAS  PubMed  Google Scholar 

  6. Riedl M, Diaz-Sanchez D. Biology of diesel exhaust effects on respiratory function. J Allergy Clin Immunol. 2005;115:221–8.

    Article  CAS  PubMed  Google Scholar 

  7. Highwood EJ, Kinnersley RP. When smoke gets in our eyes: the multiple impacts of atmospheric black carbon on climate, air quality and health. Environ Int. 2006;32:560–6.

    Article  CAS  PubMed  Google Scholar 

  8. Holt PG, Strickland DH, Wickström ME, Jahnsen FL. Regulation of immunological homeostasis in the respiratory tract. Nat Rev Immunol. 2008;8:142–52.

    Article  CAS  PubMed  Google Scholar 

  9. Vitte J, Bongrand P. Immunotoxicologie. In: Viala A, Botta A, Andrejak M, Aubert C, editors. Toxicologie. Paris: Lavoisier; 2005. p. 59–69.

    Google Scholar 

  10. Bleck B, Tse DB, Jaspers I, Curotto de Lafaille MA, Reibman J. Diesel exhaust particle-exposed human bronchial epithelial cells induce dendritic cell maturation. J Immunol. 2006;176:7431–7.

    CAS  PubMed  Google Scholar 

  11. Inoue K, Takano H, Yanagisawa R, Hirano S, Kobayashi T, Fujitani Y, et al. Effects of inhaled nanoparticles on acute lung injury induced by lipopolysaccharide in mice. Toxicology. 2007;238:99–110.

    Article  CAS  PubMed  Google Scholar 

  12. Neer A, Köylü ÜÖ. Effect of operating conditions on the size, morphology, and concentration of submicrometer particulates emitted from a diesel engine. Combust Flame. 2006;146:142–54.

    Article  CAS  Google Scholar 

  13. Smekens A, Godoi RHM, Berghmans P, Van Grieken R. Characterization of soot emitted by domestic heating, aircraft and cars using diesel or biodiesel. J Atmos Chem. 2005;52:45–62.

    Article  CAS  Google Scholar 

  14. Bartra J, Mullol J, del Cuvillo A, Davila I, Ferrer M, Jauregui I, et al. Air pollution and allergens. J Investig Allergol Clin Immunol. 2007;17S(2):3–8.

    Google Scholar 

  15. Akerman J. Sustainable air transport–on track in 2050. Transp Res Part D. 2005;10:111–26.

    Article  Google Scholar 

  16. Brasil AM, Farias TL, Carvalho MG. A recipe for image characterization of fractal-like aggregates. J Aerosol Sci. 1999;30:1379–89.

    Article  CAS  Google Scholar 

  17. Köylü ÜÖ, Faeth GM, Farias TL, Carvalho MG. Fractal and projected structure properties of soot aggregates. Combust Flame. 1995;100:621–33.

    Article  Google Scholar 

  18. Williams MA, Porter M, Horton M, Guo J, Roman J, Williams D, et al. Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naïve CD4+ T cells. J Allergy Clin Immunol. 2007;119:488–97.

    Article  CAS  PubMed  Google Scholar 

  19. Hammad H, Lambrecht BN. Dendritic cells and epithelial cells: linking innate and adaptive immunity in asthma. Nat Rev Immunol. 2008;8:193–204.

    Article  CAS  PubMed  Google Scholar 

  20. Alessandrini F, Beck-Speier I, Krappmann D, Weichenmeier I, Takenaka S, Karg E, et al. Role of oxidative stress in ultrafine particle-induced exacerbation of allergic lung inflammation. Am J Resp Crit Care Med. 2009;179:984–91.

    Article  CAS  PubMed  Google Scholar 

  21. Provoost S, Maes T, Willart MAM, Joos GF, Lambrecht BN, Tournoy KG. Diesel exhaust particles stimulate adaptive immunity by acting on pulmonary dendritic cells. J Immunol. 2010;184:426–32.

    Article  CAS  PubMed  Google Scholar 

  22. Singh PD, DeMarini M, Dick CA, Tabor DG, Ryan JV, Linak WP, et al. Sample characterization of automobile and forklift diesel exhaust particles and comparative pulmonary toxicity in mice. Environ Health Perspect. 2004;112:820–5.

    Article  CAS  PubMed  Google Scholar 

  23. Lamhamedi-Cherradi SE, Martin RE, Ito T, Kheradmand F, Corry DB, Liu YJ, et al. Fungal proteases induce Th2 polarization through limited dendritic cell maturation and reduced production of IL-12. J Immunol. 2008;180:6000–9.

    CAS  PubMed  Google Scholar 

  24. Prasse A, Germann M, Pechkovsky DV, Markert A, Verres T, Stahl M, et al. IL-10-producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients. J Allergy Clin Immunol. 2007;119:464–71.

    Article  CAS  PubMed  Google Scholar 

  25. Braun A, Bewersdorff M, Lintelmann J, Matuschek G, Jakob T, Göttlicher M, et al. Differential impact of diesel particle composition on pro-allergic dendritic function. Toxicol Sci. 2010;113:85–94.

    Article  CAS  PubMed  Google Scholar 

  26. de Haar C, Kool M, Hassing I, Bol M, Lambrecht BN. Lung dendritic cells are stimulated by ultrafine particles and play a key role in particle adjuvant activity. J Allergy Clin Immunol. 2008;121:1246–54.

    Article  PubMed  Google Scholar 

  27. Larché M. Regulatory T cells in allergy and asthma. Chest. 2007;132:1007–14.

    Article  PubMed  Google Scholar 

  28. Ouyang W, Kolls JK, Zheng Y. The biological functions of T helper 17 cell effector cytokines in inflammation. Immunity. 2008;28:454–67.

    Article  CAS  PubMed  Google Scholar 

  29. Ryan PH, Bernstein DI, Lockey J, Reponen T, Levin L, Grinshpun S, et al. Exposure to traffic-related particles and endotoxin during infancy is associated with wheezing at age three. Am J Resp Crit Care Med. 2009;180:1068–75.

    Article  CAS  PubMed  Google Scholar 

  30. Jaspers I, Sheridan PA, Zhang W, Brighton LE, Chason KD, Hua X, et al. Exacerbation of allergic inflammation in mice exposed to diesel exhaust particles prior to viral infection. Part Fibre Toxicol. 2009;6:22.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Dr. Patrick Sudour and Dr. Nathalie Charvin for helpful discussions and assistance with formal issues and Ms. Elisabeth Wostrowski and Ms. Dolores Migneret for technical assistance.

This work was supported by Assistance Publique Hôpitaux de Marseille, Institut National de la Santé Et de la Recherche Médicale, Centre National de la Recherche Scientifique, and Université de la Méditerranée.

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Authors and Affiliations

  1. Centre Interdisciplinaire de Nanoscience de Marseille CINaM CNRS UPR 3118, Université Aix-Marseille 2, Campus de Luminy, case 913, 13288, Marseille Cedex 9, France

    D. Ferry

  2. INSERM UMR 600/CNRS UMR 6212, Université Aix-Marseille 2, 13009, Marseille, France

    C. Rolland, P. Robert, P. Bongrand & Joana Vitte

  3. Atmospheric Environment Unit, ONERA, 29 Avenue de la Division Leclerc, 92320, Chatillon, France

    D. Delhaye

  4. Université Aix-Marseille 2, Pôle Cardiovasculaire et Thoracique, Hôpital Nord, Assistance Publique Hôpitaux de Marseille, Service d’Oncologie Multidisciplinaire et Innovations Thérapeutiques, Chemin des Bourrely, 13915, Marseille Cedex 20, France

    F. Barlesi

  5. Laboratoire d’Immunologie, Hôpital de la Conception, Assistance Publique Hôpitaux de Marseille, 147 boulevard Baille, 13005, Marseille, France

    P. Robert, P. Bongrand & Joana Vitte

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  1. D. Ferry
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  2. C. Rolland
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Correspondence to Joana Vitte.

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Responsible Editor: Michael Parnham.

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Ferry, D., Rolland, C., Delhaye, D. et al. Jet exhaust particles alter human dendritic cell maturation. Inflamm. Res. 60, 255–263 (2011). https://doi.org/10.1007/s00011-010-0262-9

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  • DOI: https://doi.org/10.1007/s00011-010-0262-9

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