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vol. 31 no. 165
PubMed
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History
- Received March 28, 2022
- Accepted May 16, 2022
- Published online July 27, 2022.
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Copyright ©The authors 2022 This version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. For commercial reproduction rights and permissions contact permissions@ersnet.org
Author Information
- Peter Pennitz1,16,
- Holger Kirsten2,16,
- Vincent D. Friedrich2,3,
- Emanuel Wyler4,
- Cengiz Goekeri1,5,
- Benedikt Obermayer6,
- Gitta A. Heinz7,
- Mir-Farzin Mashreghi7,8,
- Maren Büttner9,10,
- Jakob Trimpert11,
- Markus Landthaler4,12,
- Norbert Suttorp1,
- Andreas C. Hocke1,
- Stefan Hippenstiel1,
- Mario Tönnies13,
- Markus Scholz2,
- Wolfgang M. Kuebler14,15,
- Martin Witzenrath1,15,
- Katja Hoenzke1 and
- Geraldine Nouailles1⇑
- 1Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Infectious Diseases and Respiratory Medicine, Berlin, Germany
- 2University of Leipzig, Institute for Medical Informatics, Statistics, and Epidemiology, Leipzig, Germany
- 3Center for Scalable Data Analytics and Artificial Intelligence (ScaDS.AI), Leipzig, Germany
- 4Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany
- 5Cyprus International University, Faculty of Medicine, Nicosia, Cyprus
- 6Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Core Unit Bioinformatics, Berlin, Germany
- 7Deutsches Rheuma-Forschungszentrum Berlin (DRFZ), A Leibniz Institute, Therapeutic Gene Regulation, Berlin, Germany
- 8Berlin Institute of Health at Charité – Universitätsmedizin Berlin, BIH Center for Regenerative Therapies (BCRT), Berlin, Germany
- 9University of Bonn, Genomics and Immunoregulation, Life & Medical Sciences (LIMES) Institute, Bonn, Germany
- 10Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Systems Medicine, Bonn, Germany
- 11Freie Universität Berlin, Institute of Virology, Berlin, Germany
- 12Humboldt-Universität zu Berlin, Institute for Biology, IRI Life Sciences, Berlin, Germany
- 13HELIOS Clinic Emil von Behring, Department of Pneumology and Department of Thoracic Surgery, Chest Hospital Heckeshorn, Berlin, Germany
- 14Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Physiology, Berlin, Germany
- 15German Center for Lung Research (DZL), Berlin, Germany
- 16Both authors contributed equally to this work
- Corresponding author: Geraldine Nouailles (geraldine.nouailles{at}charite.de)
Funding
Bundesministerium für Bildung und Forschung
01IS18026B; 01ZX1304A; 01ZX1604B; 01ZX1906A; 01ZX1906B; 16GW0247
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Vol 31 Issue 165
Table of Contents
A pulmonologist's guide to perform and analyse cross-species single lung cell transcriptomics
Jump To
- Article
- Abstract
- Abstract
- Introduction
- Basic principles of pulmonary single-cell isolation, barcoding and sequencing
- Data processing and analysis
- Exemplary workflow for multispecies data pre-processing and integration
- Cross-species analysis of human and nonhuman scRNA-seq data
- Outlook
- Supplementary material
- Acknowledgements
- Footnotes
- References
- Figures & Data
- Info & Metrics