Abstract
The development of cystic fibrosis-related diabetes (CFRD) often leads to poorer outcomes in patients with cystic fibrosis including increases in pulmonary exacerbations, poorer lung function and early mortality. This review highlights the many factors contributing to the clinical decline seen in patients diagnosed with CFRD, highlighting the important role of nutrition, the direct effect of hyperglycaemia on the lungs, the immunomodulatory effects of high glucose levels and the potential role of genetic modifiers in CFRD.
Abstract
Cystic fibrosis (CF)-related diabetes remains one of the most important comorbidities for patients with CF because of the impact on lung function and mortality. There are numerous factors in addition to infection that contribute to the negative effects of CF-related diabetes. https://bit.ly/3nSPFW6
Introduction
Fibrocystic disease of the pancreas was first described by Anderson in 1938 [1] when pancreatic autopsy findings were finally associated with pancreatic exocrine insufficiency, arrested growth and respiratory tract infections. Many of the patients described in this cohort died from suppurative lung disease including bronchiectasis. It was then that the condition cystic fibrosis (CF) was recognised as a distinct entity. In the decades following, the life expectancy of patients with CF has improved dramatically [2] with emphasis placed on treating undernutrition and progressive lung disease because of the associated morbidity and early mortality. However, one major comorbidity which has significant clinical implications is cystic fibrosis-related diabetes (CFRD) [3, 4] because it is associated with more rapidly progressing lung disease, lung function decline and early mortality. CFRD affects approximately one-third of patients with CF over the age of 18 years [5] and, while previously considered a disease of the older child or adult, a recent paper by us and others has demonstrated that it can begin in early life [6–8]. One of the most important changes in CF management in recent years has been the introduction of screening for and treatment of CFRD [4]. However, despite these changes patients with CFRD still appear to be at greater risk of poorer nutrition and lung function, and infection with important and detrimental CF pathogens [9]. These complications are associated with an increase in lung infections and concurrent nutritional decline but there are additional pathophysiological mechanisms that link hyperglycaemia and CF-related lung disease. Here we review the literature and recent advances in the understanding of CFRD and its relationship with lung disease.
Pathophysiology of CFRD
CF results from an abnormality in the CF transmembrane conductance regulator (CFTR) gene on chromosome 7 which encodes the CFTR protein. The CFTR protein is normally situated on the apical plasma membrane of epithelial cells that line the lungs, gastrointestinal system and pancreas. It is a cyclic adenosine monophosphate (cAMP)-dependent anion channel which predominantly transports chloride and bicarbonate anions [10]. It is also known to modulate other ion channels including the epithelial sodium channel (ENaC) [11]. In patients with CF, the defective or deficient CFTR protein results in viscous secretions that in the lungs leads to a vicious cycle of neutrophilic inflammation and chronic infection. For patients with CF, this cycle ultimately leads to death from progressive lung disease and respiratory failure [12].
In the pancreas, the abnormality in the CFTR protein results in both exocrine and endocrine dysfunction. CF-related endocrine dysfunction results in elevated glucose levels, possibly because patients with CFRD have fewer insulin-secreting cells than patients with CF without diabetes [13]. “Bystander destruction” by local inspissated exocrine secretions may be the cause of the decrease in the number of insulin-secreting cells. The pancreatic endocrine tissue and insulin cells are then replaced with fibrotic tissue and deposition of amyloid [14–16]. However, the mechanism may be more complex. Some studies have suggested that glucose abnormalities in CF may be the result of primary CFTR dysfunction of the insulin-secreting beta cells of the pancreas [17–19] or dysfunction in glucagon secretion from alpha cells [20, 21]. However, not all studies are in agreement. A recent study by Hart et al. [22] showed that CFRD does occur in association with collateral exocrine (acinar) cell damage and pancreatic inflammation rather than primary CFTR dysfunction of the insulin-secreting beta cells.
There are significant consequences to the dysregulation of insulin secretion as insulin plays a crucial role in normal glucose homeostasis and skeletal muscle protein synthesis. Insulin is normally synthesised and stored in vesicles within the beta-islet cells of the pancreas. It is also produced when stimulated by a glucose load. As the primary role of insulin is to lower glucose levels, loss of beta cells and/or diminished insulin action results in hyperglycaemia. Patients with CF initially demonstrate delayed first phase insulin secretion, due to reduced exocytosis of the pre-formed insulin vesicles described above, and also blunting of overall peak insulin level [23, 24]. As a result of these changes, patients with CF develop postprandial hyperglycaemia which progresses with age towards abnormal glucose tolerance and ultimately CFRD.
Although insulin deficiency is thought to be the main cause of CFRD [25], insulin resistance does occur, as in type 2 diabetes, particularly when glucose abnormalities are severe and patients are older [26]. Insulin resistance in this setting may occur because hyperglycaemia reduces the number of glucose transporter type-4 (GLUT-4) insulin-sensitive channels expressed on cell surfaces. GLUT-4 is an insulin-regulated glucose transporter and allows facilitated diffusion of glucose into skeletal muscle and adipose tissue. The GLUT-4 channel may be downregulated in chronic hyperglycaemia causing and potentiating progressive peripheral insulin resistance [27]. This is consistent with findings in patients with CF in which their glycaemic status fluctuates in and out of a diabetic state, with glucose levels worsening at times where insulin resistance typically occurs such as during pulmonary exacerbations, glucocorticoid use, puberty and pregnancy. This cycle is further perpetuated in patients with CF with poor nutrition as they will have less adipose tissue and skeletal muscle mass to uptake glucose from the circulation.
An imbalance in free radicals and antioxidants causing oxidative stress may also play a role in the development of CFRD. Glutathione is an antioxidant that normally passes through the normal CFTR protein channel. It has been shown to be low in the airways of patients with CF [28] and low serum levels have also been associated with both type 1 and type 2 diabetes [29, 30]. Low glutathione levels result in a pro-inflammatory cascade of cytokines including IL-1 β [31] and tumour necrosis factor (TNF)-α may then further exacerbate hyperglycaemia by causing insulin resistance and inhibition of the action of insulin at the level of the receptor [32].
Clinical implications of CFRD
International CF guidelines recommend that screening for CFRD begins at 10 years of age using the oral glucose tolerance test (OGTT) [33, 34]. This test is conventionally used to diagnose type 2 diabetes based on the risk of microvascular disease complications as identified in the Pima Native American population. These diagnostic criteria for type 2 diabetes were extrapolated for use in the CF population [35]. Using these criteria, patients diagnosed with CFRD are most at risk of a significant deterioration in nutrition and lung function and have increased mortality [36]. However, more recent research has demonstrated that early glucose abnormalities may be associated with poorer clinical status in children [37] and clinical decline may actually begin several years prior to the patient meeting CFRD diagnostic criteria [38].
CFRD has also been shown to play a crucial role in the rate of lung function decline and mortality in patients with CF [38–42]. Even in patients with a normal OGTT, early glucose abnormalities have been shown to be associated with more severe lung disease. LeClerq et al. [43] used continuous glucose monitoring (CGM) to identify pre-diabetic glucose abnormalities in patients with CF with a normal OGTT. Participants in this study with elevated glucose levels on CGM had significantly lower lung function than those without glucose abnormalities and had higher rates of Pseudomonas aeruginosa. Furthermore, glucose abnormalities may hasten the progression of structural lung damage, even prior to the development of diabetes. We have previously demonstrated that in children and adolescents with CF the severity of glucose abnormalities predicted the rate of structural lung damage progression, even when there was no change in lung function identified by spirometry [44].
Diabetes and nutrition
The association between nutrition and lung function in CF has been well reported [45–49]. One of the critical functions of insulin is protein anabolism and growth in children. In insulin-deficient patients with CF, the degree of resulting catabolism has been correlated with severity of lung function deficits [50]. Milla et al. [51] have also shown that patients with CF with the lowest insulin levels during OGTT have the greatest rate of lung function decline. In children with CF, this may represent poor lung growth and failure to achieve optimal alveolarisation and vital capacity, which normally increase throughout childhood [45, 47]. This theory is supported by studies that have demonstrated the predictive capacity of paediatric nutrition on later lung function [47, 52, 53]. It could also be that poor nutrition secondary to insulin deficiency results in an increase in pulmonary exacerbation rate and lung function decline. Also, patients with CF demonstrating the greatest degree of insulin deficiency and CFRD-related protein catabolism may also have poorer respiratory muscle strength and thus effort-dependent lung function may be affected [54]. This theory is supported by as study from 1997 by Inoescu et al. [55] that demonstrated an association between low body mass index (BMI) and poorer sustained maximum inspiratory pressure (SMIP), and reduced survival. It is likely that there are multiple nutritional factors that impact on lung function.
Infection and hyperglycaemia
CF respiratory tract infections and the rate of exacerbations may be amplified by presence of glucose in the airway surface liquid (ASL). ASL glucose levels are normally tightly controlled at a concentration (approximately 0.4 mM) 12 times less than the serum level [56]. When this balance is dysregulated and there is an increase in ASL glucose, organisms such as P. aeruginosa may flourish [57]. In health, this gradient is strictly maintained by GLUT transporters, which allow diffusion of glucose into the cell to meet the cell's metabolic requirements [58] (figure 1). A second glucose sodium-coupled transporter isoform-1 (SGLT-1) is present more distally on the alveolar lumen membrane and is driven by the intracellular glucose and sodium gradients.
Schematic diagram of airway surface liquid (ASL) glucose homeostasis. a) In health, ASL has a tightly regulated glucose gradient in which minimal glucose (black triangles) is present. ASL hydration is supported by the CFTR protein which is situated on the apical plasma membrane and transports Cl− and HCO3− ions into the ASL. ASL glucose is transported to the site of intracellular glucose metabolism by GLUT transporters and there is minimal passive diffusion across tight paracellular junctions. b) In the setting of CFRD, there are changes in ASL with reduced and inflamed ASL, with an increased number of bacteria seen (crossed circles). Paracellular junctions leak glucose into the ASL. Despite initial upregulation of apical GLUT transporters, there is net increase in movement of glucose towards the ASL and an increased level of glucose is seen in the ASL. CFRD: cystic fibrosis-related diabetes; CFTR: cystic fibrosis transmembrane conductance regulator; GLUT: glucose transporter.
In the setting of inflammation, apical glucose transporters are upregulated in the lung epithelium but are nevertheless overwhelmed and unable to maintain normal ASL glucose levels [58]. This is because the usually poorly permeable tight junctions between epithelial cells begin to leak glucose into the ASL. Cellular studies in cultured immortalised epithelial cell monolayers suggest that this paracellular glucose leak results from an alteration of tight junction protein expression which appears to be critical in maintaining a paracellular junction that is impermeable to glucose [56, 59].
The elevation in ASL glucose level is one of the many mechanisms thought to contribute to the increase in pulmonary exacerbations and chronic infection with CF pathogens seen in patients who develop CFRD. An increase in bacterial growth of Staphylococcus aureus and P. aeruginosa, with increasing glucose concentrations has been shown in vitro [57]. It has also been shown that in non-CF populations, elevated ASL glucose is a risk factor for respiratory tract infections [60]. Intubated patients in intensive care are more likely to have methicillin-resistant S. aureus (MRSA) identified from bronchial aspirates if glucose levels in the aspirate are elevated. Patients with other respiratory diseases such as chronic obstructive pulmonary disease (COPD) have worse lung function and more frequent exacerbations if they also have diabetes [61, 62]. Lack of concurrent CF-related hyperglycaemia may be one differentiating factor contributing to the much lower prevalence of P. aeruginosa seen in patients with non-CF bronchiectasis when compared with patients with CF [63, 64].
Brennan et al. [57] demonstrated in a cohort of patients with CF (n=40) and healthy subjects (n=10) that blood glucose levels >8 mmol·L−1 correlate with increase in nasal ASL glucose. This is well below the diagnostic threshold for CFRD of 11.1 mmol·L−1 blood glucose. Subjects with CF were also shown to have higher ASL glucose than healthy controls, even when they had a normal OGTT. Furthermore, when the OGTT was abnormal the level of ASL glucose correlated with the severity of glucose intolerance, providing additional evidence that glucose abnormalities that precede diabetes may contribute to elevations ASL glucose. A serum glucose level of 8.2 mmol·L−1, on OGTT samples taken every 30 min, is also the threshold determined by our group that detects early nutritional and respiratory decline in patients with CF [7]. We have also shown that peak glucose level during OGTT in children with CF <10 years of age is associated with worse lung function in this young cohort [37].
Further evidence comes from Garnett and colleagues who demonstrated that elevations in glucose promoted the growth of P. aeruginosa on primary CF and non-CF human bronchial epithelial cell monolayers [65]. The extent of bacterial growth was greater on the CF cells than the controls. Glucose elevation had a greater impact on P. aeruginosa growth than any of the other CFTR mechanisms tested including mucus hyperviscosity and reduced fluid volume. Furthermore, P. aeruginosa filtrate appeared to decrease transepithelial resistance resulting in greater glucose flux across the CF monolayer, thus setting up a vicious cycle of elevated glucose perpetuating bacterial growth which further increases glucose levels on the apical monolayer.
Hyperglycaemia and the pulmonary microbiome
Given the evidence to support a role for hyperglycaemia having an effect on growth of respiratory pathogens detected by culture it is therefore not unexpected to find that the pulmonary microbiome may also be altered by diabetes [66]. However, the impact of diabetes on the bacterial milieu in patients with CF when evaluated by nonculture methods is yet to be fully elucidated. Studies have shown that the pulmonary microbiome of patients with CF decreases in diversity with age and correlates with severity of clinical disease [67]. It is not yet known though what factors are the primary drivers of the changes seen in the lung microbiome diversity and whether or not the evolution of glucose abnormalities is one contributing driver of this dysbiosis. Studies which will attempt to answer this question will be fraught as significant confounders will include the increase in exacerbation frequency and thus antibiotic usage in this cohort. There are as yet no studies to our knowledge that evaluate the link between pre-diabetic glucose abnormalities and the early pulmonary microbiome in CF.
Tissue damage
Patients with diabetes, without a primary respiratory condition or diagnosis, have been shown to have histological changes in the lungs. Hsia et al. [68] demonstrated parenchymal changes including thickened basement membranes and septa and fibrosis. This evidence was supported by findings of poorer lung function in patients with type 1 [69, 70] and type 2 diabetes [71–73] that are not explained by BMI alone. Yeh et al. [74] studied over 10 000 adults, 1100 with type 2 diabetes, and found that they had significantly lower lung function than those without diabetes. The systematic review undertaken by Klein et al. [75] highlighted the reduced forced vital capacity (FVC) and forced expiratory volume in 1 s (FEV1), two objective measures of lung function measured by spirometry, in patients with diabetes when compared with nondiabetic controls. A smaller study by Ledesma Velázquez did not identify a similar relationship with FEV1, FVC and FEV1/FVC, in the diabetic, pre-diabetic and euglycaemic groups but did identify a lower peak expiratory flow rate in diabetic patients [76]. However, fasting serum glucose was associated with a decrease in FEV1 and FEV1/FVC.
Several other studies have reported an inverse correlation between hyperglycaemia and lung function. Yang et al. [77] examined the association between glycated haemoglobin A1c (HbA1c), asthma-related hospitalisations and lung function in 47 606 adults aged 40 to 69 years in their cross-sectional study. They found an inverse association between lung function (FEV1 and FVC) and HbA1c level, even when HbA1c was not within the diabetes/pre-diabetes range. This is not dissimilar to the study undertaken by Oh et al. [78] who identified a relationship between HbA1c and FVC and FEV1 in 3670 participants without diabetes or known lung disease. Logistic regression analysis undertaken in this study revealed a significant association between HbA1c and a restrictive pattern on spirometry (OR 3.772). Uz-Zaman et al. [79] found that diabetic patients had a reduced FEV1, FVC and FEV1/FVC when compared with healthy controls but also identified a reduction in diffusion capacity (diffusing capacity of the lung for carbon monoxide (DLCO) % and DLCO/alveolar volume %). They hypothesised that the reduction in diffusion capacity could be due to “nonenzymatic glycosylation” and “chronic diabetic microangiopathy causing basement membrane thickening … leading to reduction in strength and elasticity of connective tissues and reduced pulmonary blood volume with V/Q [ventilator/flow] mismatch”. Further evidence of the impact of hyperglycaemia on lung function comes from Gutiérrez-Carrasquilla who led the Sweet Breath study which examined the lung function of 60 adult participants with type 2 diabetes before and after treatment “intensification” to improve glycaemic control [80]. Participants who responded to their diabetes treatment (defined as reduction in their HbA1c of ≥0.5%) showed evidence of improvements in both their FVC and FEV1. The absolute change in HbA1c was also inversely correlated to the increases in FEV1.
These lung function results are not dissimilar to studies of patients with CF with early glucose abnormalities [7]. In a study of 33 children with CF, in which OGTT and CGM were performed, we also identified declining FVC and FEV1 with increasing severity of glucose abnormalities on CGM [7]. Several mechanisms have been proposed including: microangiopathy of small pulmonary vessels, chronic local inflammation, loss of elastic recoil secondary to collagen glycosylation and local insulin resistance secondary to hypoxia [75]. Hyperglycaemia is known to result in glycosylation of serum and tissue proteins resulting in the formation of advanced glycosylation end products. These products cause inflammation and lead to complications in the kidneys and eyes, and it is thus not unexpected that the extensive alveolar-capillary network within the lung may also be affected by microangiopathy.
ASL acid–base balance
Evidence regarding the contribution of ASL pH towards the development of CF-related lung disease remains inconclusive [81]. CFTR dysfunction results in reduced bicarbonate transport which contributes to hyperviscosity and dehydration of the ASL [82–84]. Abou Alaiwa et al. [85] identified a more acidic nasal pH in seven neonates with CF when compared with controls. Consistent with this, the study performed by Pezzulo et al. [86] using newborn CF pigs was also able to show ASL to be more acidic in vivo and in primary airway epithelial cell culture when compared with non-CF littermates. The decrease in pH in the latter study was associated with impaired bacterial killing that was restored by increasing the pH. This may be one mechanism that contributes to the cycle of recurrent infections and is supported by in vitro experiments using primary airway epithelial cells undertaken by Nakayama et al. [87]. This is in contrast to the study by Schultz et al. [88] which examined ASL pH in children with CF during bronchoscopy. This study showed that airway pH in children with CF was no different from that of the controls. One study by Garnett et al. [89] demonstrated that CF human bronchoepithelial (HBE) cells secrete lactate in the setting of hyperglycaemia resulting in a more acidic ASL. In the presence of P. aeruginosa, the acidosis effect of hyperglycaemia on CF HBE cells was further amplified [89]. We propose that this may be one factor related to developing lung disease that explains the negative results described by Schulz et al. [88] whose participants were young but also fasted for their procedure and were thus less likely to have elevated serum glucose levels and concurrent P. aeruginosa infection at that age.
Hyperglycaemia and the effect on the inflammatory response
CF lung disease results from a persistent and unrelenting inflammatory response characterised by neutrophilic inflammation and recurrent infection. Bacterial infection leads to oxidative stress which propagates the inflammatory response. Numerous studies have demonstrated pro-inflammatory cytokines in the lungs of patients with CF, including IL-1β, IL-6 and IL-8 and tumour necrosis factor-α as reviewed by Roesch et al. [90]. Conversely anti-inflammatory cytokines such as IL-10 have been shown to be downregulated in the respiratory epithelium in patients with CF. However, the question of whether inflammation occurs without infection has not been definitively answered and it is not yet clear whether infection precedes the inflammatory process in young children with CF [91, 92]. The degree of inflammation is not entirely explained by the presence of bacteria alone and it is possible that CF cells exist in a heightened inflammatory state. In studies that compare children with CF to children with noncystic fibrosis bronchiectasis, patients with CF have a significantly higher pulmonary neutrophil burden than those with non-CF bronchiectasis, even when infection is taken into account [93].
Montongomery et al. [94] have also identified an association between IL-1 (including IL-1 β) and neutrophils, neutrophil elastase activity in bronchoalveolar lavage fluid and structural lung disease in young children with CF. IL-1 is also associated with CFRD. Hull et al. [95] demonstrated that islet IL-1β immunoreactivity was elevated in pancreatic autopsy specimens of patients with CF and CFRD. Further research needs to be undertaken to determine if pancreatic inflammation has a direct impact on the pulmonary inflammatory process and pulmonary disease via the systemic release of inflammatory mediators such as IL-1. The reverse could also be true, namely that pulmonary or sinus inflammation could release cytokines into the systemic circulation that results in islet cell damage. This relationship is particularly important as a potential therapeutic target given that anti-IL-1 antibodies are already in established clinical trials [96] with some in clinical use for immunological conditions such as anakinra (KineretTM; Amgen).
Perhaps the driving force of pulmonary inflammation is elevated glucose rather than infection. CFTR-knockout ferrets treated with long-term antibiotics to prevent infection from birth continue to demonstrate a prominent pulmonary inflammatory response [97]. Additional studies also report altered neutrophil chemotaxis and function in the setting of hyperglycaemia. Hunt et al. [98] reported CF-diabetic mice fail to clear inoculated P. aeruginosa when compared with CF-nondiabetic mice and controls [98]. This was despite an appropriate and augmented pulmonary neutrophilic response. Research performed by our team supports the role of hyperglycaemia in the pulmonary inflammatory process. In one study performed by our research group, a significant correlation between the degree of hyperglycaemia on CGM and pulmonary inflammation (neutrophilia and IL-8) in bronchoalveolar lavage was detected in children with CF <6 years of age [8]. This association was seen with glucose levels well below that of patients with CF diagnosed with CFRD.
CF inflammation occurs as a result of recruitment and activation of polymorphonuclear neutrophils (PMNS) and their release of toxic intracellular granules filled with neutrophil elastase, myeloperoxidase and other mediators into the nearby extracellular space. Neutrophil elastase, in particular has been shown to play a key role in the development of bronchiectasis in its association with structural lung damage in young children with CF [99]. Metalloproteinases and other neutrophilic contents have also been associated with pulmonary damage in children with CF [100]. One potential mechanism lies with the neutrophil's release of NETs (neutrophil extracellular traps) [101]. NETs are thought to contain several mediators associated with bronchiectasis in CF including neutrophil elastase and matrix metalloproteinase [102]. One study by Wong et al. [103] demonstrated that NETs are released at the onset of diabetes resulting in rupture of the neutrophil contents. Joshi et al. [104] noted in their study that the neutrophils exposed to a hyperglycaemic environment formed NETs without external stimulation (e.g. lipopolysaccharide stimulus); however, it is important to note that these studies were performed in cells from healthy volunteers and diabetic patients, not from neutrophils attained from patients with CF. The NETs of these studies were smaller, showed greater instability and disintegrated rapidly suggesting that in a diabetic environment the neutrophils are constitutively active and may be less efficient. However, the authors are not aware of any studies that have evaluated the link between NETs in the evolution of CFRD-related lung damage at this time.
Immunomodulation
Hyperglycaemia may have an impact on the immune system and one such mechanism is via resistin release. Resistin is an immunometabolic mediator that has been shown to be elevated in inflammatory conditions including arthritis, asthma and cardiovascular disease. It has been implicated in the relationship between obesity and type 2 diabetes [105]. Resistin modulates inflammation by binding lipopolysaccharide (LPS) receptor toll-like receptor 4 (TLR4) which modulates activation of B-cells, protein kinase signalling and cytokine secretion [106, 107]. Nagaev et al. [107] have shown that the addition of resistin to adipose tissue samples resulted in stimulation of inflammatory cytokines including IL-6 and IL-8. Forrest et al. [108] have recently demonstrated elevated resistin levels in CF sputum at the onset of pulmonary exacerbations (100 times higher in patients with CF compared with controls), and also established a negative correlation with lung function (Rho −0.78, p=0.001). Furthermore, elevated resistin levels in sputum were associated with CFRD during inpatient admission and sputum resistin levels were positively correlated with number of de-granulated (i.e. post NET release) PMNs [108].
Variation in the innate immune system function, specifically mannose-binding lectin (MBL) may be another determinant of outcomes in patients with CF [109–111]. Gravina et al. [112] evaluated MBL2 variants in approximately 100 children with CF in Argentina. In this study, patients with CF with MBL insufficiency were at greater risk of having a severe phenotype and earlier onset of P. aeruginosa infection [112]. These study findings are further supported by Garred et al. who also found that the predicted age of survival was reduced by 8 years in patients with MBL variant alleles [111]. The results of a study by Ilyas et al. [113] may provide an important clue that perhaps innate immune dysfunction in patients with CF may not be limited only to patients with low MBL levels. Ilyas et al. [113] evaluated the carbohydrate binding capacity of the lectin pathway (including MBL) in the presence of variable glucose levels. Lectin function was disrupted by competitive inhibition and complement activation was found to be inhibited in the presence of high glucose. Further studies need to be conducted to evaluate the function of the lectin pathway in patients with CFRD and patients with CF with early glucose abnormalities, but this is one biologically plausible mechanism that may contribute to recurrent infections in CF.
Receptor for advanced glycation end products
The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily of cell surface molecules [114]. It is a multiligand receptor and regulates chronic inflammation and immune responses [115]. RAGE production potentiates downstream production of inflammatory cytokines, adhesion molecules and matrix metalloproteinases. It is present in the lungs and on inflammatory cells including neutrophils, macrophages, monocytes and lymphocytes [116]. RAGE has been linked to CF and also to diabetes. Patients with CF have been shown to have elevated RAGE on airway neutrophils. Patients with CF also have increased levels of enRAGE (S100A12, extracellular newly identified receptor for advanced glycation end-products) and lower levels of defensive sRAGE (soluble receptor for advanced glycation end-products) in the ASL. Diabetes is also associated with elevated advanced glycation end products (AGE) which have been shown to upregulate inflammation via upregulation of RAGE.
Mulrennan et al. [114] evaluated the relationship between RAGE and CFRD in a study which assessed different forms of RAGE in serum, white blood cells and sputum of patients with CF, diabetes and CFRD and healthy controls. This study evaluated the ratio between S100A12 enRAGE, (secreted by activated granulocytes and previously shown to be elevated in CF), and sRAGE (which mitigates inflammation by binding pro-inflammatory ligands). In healthy adults, sRAGE and enRAGE are balanced [117]. Decreased sRAGE is associated with atherosclerosis, arthritis and CF and diabetes. In the study by Mulrennan et al. [114], sputum enRAGE/sRAGE ratios were elevated in patients with CF, and particularly high in those with CFRD. The elevated RAGE ratios negatively correlated with lung function (FEV1), thus providing one potential mechanism by which hyperglycaemia contributes to the inflammatory cascade in CF. This finding is supported by the study by Hunt et al. [118] who demonstrated significantly elevated AGE and enRAGE levels in CFRD which also negatively correlated with FEV1. However, in this study CFRD patients had normal levels of “decoy sRAGE”, further implicating RAGE activation in patients with CFRD and a pro-inflammatory mechanism for lung disease.
High-mobility group box-protein (HMGB1), is one ligand that is bound by RAGE. Montanini et al. [119] evaluated the role of HMGB1 in CFRD. They were able to show that HMGB1 levels were increased at the onset of CFRD in 43 patients with CF, and that elevated HMGB1 correlated with fasting insulin:glucose ratio and area under the curve for insulin. They also showed in a concurrent in vitro study that human bronchoepithelial cells with loss of CFTR function had increased HMGB1 levels that were corrected with exogenous insulin.
CFRD genetic modifiers and the relationship with lung disease
Certain patients with CF are predisposed to develop CFRD and the consequent effects. Soave et al. [120] identified an association between single nucleotide polymorphisms (SNPs) in the SLC26A9 gene in patients with CF and risk of CFRD. This gene encodes an epithelial bicarbonate and chloride channel protein that has been shown to interact with the CFTR increasing the risk of intestinal obstruction, but has also been shown to modify lung function in patients with gating mutations and appears to explain the variable response to CFTR modifier therapies [121]. It is possible that the genetic modifier that increases the risk of CFRD also has implications within the lung and that this solute carrier will potentiate CF lung disease via the creation of further electrolyte and fluid imbalances [122]. The importance of type 2 diabetes modifiers is supported by a more recent study undertaken by Aksit et al. [123] who confirmed the association between type 2 diabetes polygenic risk scores and risk of developing CFRD in patients with CF.
Future research avenues
Exogenous insulin is currently the only recommended treatment for CFRD with evidence that treatment based on 2-hour OGTT criteria can improve lung function, nutritional status, exacerbation frequency and life expectancy [124]. Oral hypoglycaemic agents have not been routinely used in CFRD because there is not enough evidence of benefit, and there are potential concerns of accelerated beta cell loss [125]. Traditionally used in the setting of insulin resistance, such as in type 2 diabetes rather than insulin deficiency, concerns have been raised regarding the use of oral agents in CFRD specifically because of their negative side-effect profiles. Given the treatment burden of patients with CF who then also need to start insulin injections when CFRD is diagnosed, oral hypoglycaemics agents are an attractive alternative option; however, concerns about weight loss, gastrointestinal symptoms and liver dysfunction make their routine use in challenging in patients with CF. As patients with CF are now living longer, it appears that treatment of insulin resistance may also be important [126]. There is also in vitro evidence that metformin, an oral insulin sensitiser agent, may mitigate the paracellular glucose flux across the epithelial cell tight junctions and resultant increase in bacterial growth, which would be very useful in the setting of lung disease with concurrent diabetes if shown to be useful clinically [59]. Moreover, metformin treatment appears to inhibit the ENaC channel which results in slowing of apical fluid reabsorption in vitro, and appears to decrease the section of pro-inflammatory cytokines in immortalised CF human bronchial epithelial cell layers [127]. With emerging evidence that metformin can be well tolerated in some patients with CF [128], further studies are warranted to evaluate the clinical utility of this medication in CFRD. Finally, given the increasing life expectancy of patients with CF and the published data showing an increase in the proportion of patients with CF who are overweight or obese [129], more research is needed to determine if there is a subgroup of patients with CFRD who may benefit from newer oral hypoglycaemic agents under investigation such as incretin analogues.
Looking to the future, perhaps specific treatment for CFRD may not be needed if CFTR modifiers are initiated early and protect the endocrine and exocrine pancreas from inflammation and resultant destruction. Even though more recent evidence suggests that CFTR may not be present in the beta cells of the pancreas and that dysglycaemia may occur secondary to changes in the inflammatory milieu of the pancreas [22], there does appear to be some modest evidence that modifiers improve glucose abnormalities [130–133]. However, longitudinal CFTR modifier studies need to be undertaken with glucose abnormalities as the primary outcome to determine if new modifiers treat, slow the onset or even prevent CFRD.
Conclusion
It is clear that CFRD plays a crucial role in clinical outcomes for patients with CF. Increasing evidence suggests that nondiabetic early glucose abnormalities may also be important in lung function and nutrition and must be identified in order to optimise patient outcomes. All of the mechanisms driving the deterioration in lung function and increase in structural lung damage may not yet have been elucidated but so far the evidence suggests that the disease progression is likely to be multifactorial (figure 2). There are several potential mechanisms that are biologically plausible and evidence from the non-CF literature suggests that immune function may be altered by hyperglycaemia resulting in an ineffective and frustrated pulmonary inflammatory response in patients with CF. With an increasing number of CFTR modifiers with ever improving efficacy becoming available for patients with CF, it will be important to ensure that future treatments targeting the CFTR protein will also treat evolving glucose abnormalities and CFRD. As yet, very few studies have examined glucose tolerance as a primary outcome in CFTR trials and patients may be at risk of ongoing pulmonary complications if endocrine dysfunction is not concurrently addressed.
Graphical representation of potential mechanisms involved in the loss of lung function associated with the development of cystic fibrosis-related diabetes (CFRD). The lung function effects of CFRD are likely to be multifactorial with some mechanisms having greater influence in certain patients.
Footnotes
Provenance: Submitted article, peer reviewed
Conflict of interest: B.J. Prentice reports other funding from Vertex/TSANZ fellowship, NHMRC postgraduate scholarship, and the Australian Government Research Training Program scholarship, during the conduct of the study.
Conflict of interest: A. Jaffe has nothing to disclose.
Conflict of interest: S. Hameed reports grants from National Health and Medical Research Council of Australia, Australasian Cystic Fibrosis Research Trust, Regional Diabetes Support Scheme, Sydney Children's Hospital Foundation, and Australasian Pediatric Endocrine Care Grant from Pfizer, and non-financial support from Novo Nordisk, Medtronic and Abbott Diagnostics, during the conduct of the study.
Conflict of interest: C.F. Verge reports grants from National Health and Medical Research Council of Australia, Australasian Cystic Fibrosis Research Trust, Regional Diabetes Support Scheme, Sydney Children's Hospital Foundation and Australasian Pediatric Endocrine Care Grant from Pfizer, and non-financial support from Novo Nordisk, Medtronic and Abbott Diagnostics, during the conduct of the study.
Conflict of interest: S. Waters has nothing to disclose.
Conflict of interest: J. Widger has nothing to disclose.
- Received September 4, 2020.
- Accepted November 13, 2020.
- Copyright ©ERS 2021
This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.