… During wound repair, trefoiled peptides, such as TFF2, exhibit a synergistic effect with EGF
and enhance epithelial migration by activation of the protein kinase-C and extracellular signal…

In healthy subjects, the respiratory epithelium forms a continuous lining to the airways and to
the environment, and plays a unique role as a barrier against external deleterious agents to …

… Members of mir-449 family of mirNa (yellow hairpins) are strongly enriched in multiciliated
cells while their level of expression is nearly undetectable in other cell types (C) Model …

… (antagomiR), 5′-a s c s c s agcuaacaauacacugc s c s a-Chol-3′ and Notch1 protector …
The negative control was the Clear-miR (5′-c s a s u s cgucgaucguagcg s c s a-Chol-3′) …

… Air–liquid interface cultures were rinsed in PBS and fixed for 60 min at 4C in 2.5%
glutaraldehyde-PBS. After several washes with PBS and S-collidin (Sigma)-HCl buffer, pH 7.8, the …

… (C) Detection of keratinocyte-specific gene expression by RT-PCR … Vitamin C regulates
keratinocyte viability, epidermal barrier, and basement membrane in vitro, and reduces wound …

… Tubes were incubated in the thermocycler for 20 minutes at 30C and then 1 minute at 90C
followed by 30 cycles of 30 seconds/92C, 30 seconds/52C, and 30 seconds/72C. After …

… Reverse transcription was performed at 70C for 15 minutes. Amplification cycles were as
follows: 15 seconds at 94C, 15 seconds at 68C, 10 seconds at 72C. Twenty-five cycles were …

… Cells were cultured at 37 C up to confluency, then detached and counted. … (C) No cell
proliferation was seen at step I in non-CF and CF epithelia while it increased drastically at step II (p …

… incubation at 4C with 1 μmol/L αBTX-Alexa Fluor 488 conjugate, 2) fixation with 4%
paraformaldehyde for 20 minutes at room temperature, 3) overnight incubation at 4C with 5 μg/ml …