Chest
Laboratory and Animal InvestigationsNeutrophil Chemotactic Activity of Sputum From Patients With COPD: Role of Interleukin 8 and Leukotriene B4
Section snippets
Patients
Twenty patients with diagnosed COPD underwent sputum induction. All patients fulfilled the diagnostic criteria of the British Thoracic Society.22 None of the patients had actively smoked for at least 6 months prior to the study; received inhaled or oral corticosteroids, or other systemic drugs including theophylline, antibiotics or nonsteroidal anti-inflammatory drugs; or reported any acute exacerbations for at least 4 weeks prior to sputum induction. The study was approved by local regulatory
Concentration of Chemoattractants in Pooled Sputum Supernatant
The concentration of IL-8 and LTB4 in the pooled sputum supernatant was 20.9 ng/mL and 12.6 pg/mL, respectively.
IL-8 and LTB4-Induced Neutrophil Chemotaxis
Under resting conditions or after exposure to diluent, human neutrophils underwent spontaneous chemotaxis affecting 0.4 ± 0.2% of the total cell population after 1 h. The addition of IL-8 and LTB4 augmented chemotaxis over the spontaneous response in a concentration-dependent manner with the effective concentration causing a 50% fall (EC50) values of 4 ng/mL and 3.3 nmol, respectively
Discussion
Neutrophilic inflammation is an important feature of COPD and may contribute to mucus hypersecretion and destruction of alveoli. Several factors have been identified as chemoattractants for peripheral blood neutrophils, which may result in an increased trafficking of these cells to bronchial tissues. The present study was designed to assess the contribution of IL-8 and LTB4 present in the sputum of patients with established COPD on sputum-induced neutrophil chemotaxis.
The methodology was
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