Determination of CFTR chloride channel activity and pharmacology using radiotracer flux methods

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Abstract

Flux studies using either radioisotopes or ion-selective electrodes are a convenient method to assay the function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl channel. Here, we described three different protocols to study the properties, regulation and pharmacology of the CFTR Cl channel in populations of cells and artificial vesicles. These techniques are widely used to evaluate the function of wild-type and mutant CFTR prior to detailed analyses using the patch-clamp technique. Moreover, they have proved especially valuable in the search for new drugs to treat cystic fibrosis.

Keywords

CFTR
Chloride channel activity
Iodide efflux
Chloride efflux
Ion-selective electrode

Abbreviations

cpm
counts per minute
IBMX
isobutylmethylxanthine
PC
phosphatidylcholine
PE
phosphatidylethanolamine
PS
phosphatidylserine

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