Asthma, Rhinitis, Other Respiratory DiseasesLeukotrienes and 8-isoprostane in exhaled breath condensate of children with stable and unstable asthma☆
Section snippets
Study subjects
The study included 14 steroid-naive children with mild persistent asthma, 13 ICS-treated children with stable mild-to-moderate persistent asthma, and 9 children with unstable asthma (Table 1). Cases were recruited among patients attending the Pulmonology/Allergy Outpatient Clinic at the Pediatrics Department in Padova. The diagnosis of asthma was based on clinical history and examination, pulmonary function parameters, and response to β2-agonist agents, according to international guidelines.18
Cys-LTs
In the three groups of asthmatic children, EBC concentrations of cys-LTs were significantly higher than those detected in healthy control children (steroid-naive asthmatic children: median, 10.8 pg/mL [IQR, 9.3 to 13.1],P < .001; ICS-treated children with stable asthma: 12.7 pg/mL [9.9 to 15.1], P < .001; children with unstable asthma: 106.0 pg/mL [4.2 to 218.0], P < .01; healthy control children: 4.3 pg/mL [2.0 to 8.3]; Fig 1 and Table I). Cys-LTs levels were increased in children with
Discussion
Our study shows that cys-LT and 8-isoprostane concentrations in EBC are significantly higher in asthmatic children than in healthy control children. Cys-LT levels were higher in children with unstable asthma than in steroid-naive and ICS-treated children with stable asthma. FENO levels were also higher in asthmatic children, and more so in steroid-naive and unstable patients than in ICS-treated stable children.
Cys-LTs appear to have a central role in asthma pathophysiology. They mediate several
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Supported in part by a grant of Regione Veneto, Ricerche Sanitarie Finalizzate (No. 885/03/99).