Enhanced human IgE production results from exposure to the aromatic hydrocarbons from diesel exhaust: Direct effects on B-cell IgE production☆,☆☆,★,★★
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Cell preparation and culture conditions
PBMCs were obtained from healthy donors between the ages of 21 and 48 years. Tonsil cells were obtained from specimens removed at surgery, and single cell suspensions were prepared. Mononuclear cells were obtained by density-gradient centrifugation with Lymphoprep (Nycomed Pharma, Oslo, Norway; 1077 kg/m3). For complete T cell removal, two cycles of 2-aminoethylisothiouronium bromide hydrobromide (Sigma Chemical Co., St. Louis, Mo.) treated sheep red cell rosette formation and depletion were
PAH-DEP enhances IgE production induced by IL-4/CD40
We tested the ability of PAH-DEP to induce human IgE production. When added alone over a dose range of 0.005 to 500 ng/ml, PAH did not induce de novo IgE production from either PBMCs or purified human B cells. Similarly, PAH-DEP was unable to induce IgE production from PBMCs or purified human B cells in the presence of CD40 mAb. When purified B cells were treated with IL-4 to induce ϵ germline transcription,28 addition of PAH-DEP still did not lead to the appearance of IgE protein in the
DISCUSSION
In this report we demonstrate that PAH-DEP enhances the production of IgE from human B cells. Production of IgE from human cells involves a number of distinct steps: (1) induction of ϵ germline transcription, (2) DNA recombination (isotype switching), (3) RNA transcription, (4) RNA splicing, and (5) translation of the mature mRNA into protein.20, 28, 31, 32 PAH-DEP–enhanced IgE production was not attributable to induction of germline transcription or isotype switching. PAH-DEP was unable to
Acknowledgements
We thank Drs. Eugene Medlock and Larry Sousa of Amgen, Inc. (Thousand Oaks, Calif.) for the generous gift of recombinant IL-4 and Dr. Ed A. Clark of the University of Washington, Seattle, Washington, for the kind gift of anti-CD40 mAb G28-5.
References (48)
- et al.
Adjuvant activity of diesel-exhaust particulates for the production of IgE antibody in mice
J ALLERGY CLIN IMMUNOL
(1986) - et al.
Diesel-exhaust particulates inoculated by the intranasal route have an adjuvant activity for IgE production in mice
J ALLERGY CLIN IMMUNOL
(1987) - et al.
A review of 2,3,7,8-tetrachlorobenzo-p-dioxin induced changes in immunocompetence: 1991 update
Toxicology
(1991) - et al.
Sensitivity to suppression of cytotoxic T cell generation by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is dependent on the Ah genotype of the murine host
Toxicol Appl Pharmacol
(1984) - et al.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) enhances antibody production and protein kinase activity in murine B cells
Biochem Biophys Res Comm
(1987) Selection procedures both for and against cells possessing cytochrome P45OIA1-dependent aryl hydrocarbon hydroxylase activity
- et al.
Genetic and molecular analysis of the Ah receptor and of Cyplal gene expression
Biochimie
(1991) - et al.
Detection and characterization of Ah receptor in tissue and cells from human tonsils
Toxicol Appl Pharmacol
(1991) - et al.
Direct effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on responses to lipopolysacharide (LPS) by isolated murine B-cells
Immunopharmacology
(1993) - et al.
Effects of cytochrome P1-450 inducers on the cell-surface receptors for epidermal growth factor, phorbol 12,13-dibutryate, or insulin of cultured mouse hepatoma cells
J Biol Chem
(1983)
The relation between polycyclic aromatic compounds in diesel fuels and exhaust particulates
Fuel
Factors influencing the composition of the organic fraction of diesel particulates
J Aerosol Sci
Serum IgE in nonatopic smokers, nonsmokers, and recent exsmokers: relation to lung function, airway symptoms, and atopic predisposition
J ALLERGY CLIN IMMUNOL
Epidemiology of the allergic response
Influence of environmental factors on IgE production. IgE, mast cells and the allergic response
Ciba Found Symp
Hay fever, a post industrial revolution epidemic: a history of its growth during the 19th century
Clin Allergy
Studies of prevalence of Japanese cedar pollinosis among the residents in densely cultivated area
Ann Allergy
Health aspects of diesel exhaust particulate emissions
Bull NY Acad Med
Nasal allergy and air pollution
Oto-Rhino-Laryngol Tokyo
John Bostock, hay fever, and the mechanism of allergy
Lancet
Enhancing effect of suspended particulate matter on the IgE antibody production in mice
Int Arch Allergy Appl Immunol
Suppression of humoral antibody production by exposure to 1,2,3,6,7,8-hexachlorodibenzo-p-diozin
J Pharmacol Exp Ther
Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on humoral immunity. I. Similarities to Staphylococcus aureus Cowan Strain I (SAC) in the in vitro T-dependent antibody response
Immunopharmacology
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From aThe Hart and Louise Lyon Laboratory, Division of Clinical Immunology/Allergy, Department of Medicine; bThe Molecular Biology Institute; and cThe Jonsson Comprehensive Cancer Center, UCLA School of Medicine, University of California, Los Angeles.
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Supported by United States Public Health Service grants AI-15251 and AI-34567 (UCLA Asthma, Allergy and Immunologic Disease Center), and gifts from the Asthma Research Foundation and the Dorothy Fund. David Diaz-Sanchez is the recipient of the McClure Foundation Fellowship from the Los Angeles Chapter of the Asthma and Allergy Foundation.
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Reprint requests: Andrew Saxon, MD, Division of Clinical Immunology/Allergy, Department of Medicine, 52-175 Center for Health Sciences, UCLA School of Medicine, Los Angeles, CA 90024-1680.
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