Regular Article
Clearance of Circulating Endothelin-1 by ETB Receptors in Rats

https://doi.org/10.1006/bbrc.1994.1395Get rights and content

Abstract

Exogenous endothelin (ET) is rapidly cleared from the circulation. We investigated which ET receptor subtypes (ETA and ETB) participate in ET-1 clearance. Following an intravenous (i.v.) bolus dose of [125I]ET-1 in anesthetized rats, radioactivity was rapidly cleared from the circulation and trapped by the lungs, kidneys and liver. Tissue distribution of the radioactivity was significantly inhibited in the lungs and kidneys, but not in the liver by infusion of the ETB antagonist BQ-788 (0.1 mg/kg/min i.v.), and the ET-1 clearance rate was reduced, while the ETA antagonist BQ-123 had no such effect. Furthermore, in isolated perfused rat lungs, about 80% of bolus-injected [125I]ET-1 was retained by the lungs after one passage. The retention of ET-1 was significantly inhibited by infusion of 1 μM BQ-788, but not BQ-123. These results suggest that ETB receptors play an important role in the clearance of ET-1.

References (0)

Cited by (605)

  • Endothelins (EDN1, EDN2, EDN3) and their receptors (EDNRA, EDNRB, EDNRB2) in chickens: Functional analysis and tissue distribution

    2019, General and Comparative Endocrinology
    Citation Excerpt :

    In chicken liver, both cEDNRB and cEDNRB2 are highly expressed, where EDNRB/B2 signaling may be an important regulator of sinusoid vascular resistance, as reported in mice (Ling et al., 2012). Since the three EDNs are weakly expressed in the liver, therefore, it is tempting to speculate that as in rats, liver EDNRBs may also function as clearing receptor(s) to remove circulating EDN in chickens (Fukuroda et al., 1994). Of particular interest to note is that EDN2 has the highest expression level in many parts of the GI tract, including the proventriculus, jejunum, ileum, cecum and colon (Fig. 7E).

View all citing articles on Scopus
View full text