Pleural infection is a disease of historical importance and is still a modern menace, with incidences rising in adults and children, and a significant mortality in adults. Basic research is hampered by limitations with in vivo models, and the bacteriology of empyema is complex. The role of thoracic ultrasound in guiding investigation and drainage of empyema is clear. Prompt treatment with appropriate systemic antibiotics and chest tube drainage are the key; in cases of failure of these measures, thoracic surgery is of proven efficacy in the treatment of this age-old disease.
Pleural infection is one of the oldest and severest diseases. Drainage of the pleural cavity was attempted by Hippocrates over 2,000 years ago to treat empyema. During the influenza pandemic of 1917–1919, closed pleural drainage became widely practiced to treat post-pneumonic empyema . Throughout history, many famous physicians, such as Dr Guillaume Dupuytrens and Sir William Osler, have died from empyemas. Rapid recognition of the development of empyema is crucial to successful treatment; even with appropriate therapeutic attempts, the mortality of patients with empyema is 15–20% [2–4] and higher in immunocompromised patients .
The definition of empyema is pus in the pleural space , although most clinical trials and studies use the term pleural infection to encompass both empyemas and complicated parapneumonic effusions. It can affect any age group, sex and ethnicity and over 65,000 patients suffer from a pleural infection each year in the UK and USA , with an estimated hospital cost of 500 million USD. The incidence of empyema is rising in both developed and developing countries, including in paediatric populations. In Scotland, the incidence of empyema has risen 10 times in 1–4 yr old children since 1998 , with similar reports from the USA, Canada and elsewhere in Europe, and the trend is mirrored in adults [9–12]. The prevalence of HIV/AIDS, wider use of immunosuppressants and organ transplantation, and increasing age of the population mean pleural infection will continue to remain a common and significant illness.
Pleural infection can also complicate thoracic (open or closed) trauma and iatrogenic procedures, such as surgery (especially lung resection) or oesophageal rupture. “Spontaneous” bacterial empyema is rare. It is possible that genetic predisposition contributes to development of empyema in some patients . Despite numerous studies the best management of pleural infection remains controversial with widespread variations in practice .
This review will examine novel themes of translational research, highlighting areas of clinical importance and fields in need of further investigation, as well as evaluating the current best clinical management of pleural infection.
Animal models of pleural infection
Pleural infection remains difficult to treat in part, as the underlying disease mechanisms are poorly elucidated. Basic laboratory research in pleural infection is hampered by a lack of suitable in vivo models. Attempts have been made using a variety of species, including mice, rats, rabbits, guinea pigs and sheep, to develop a clinically reliable model of empyema. Many models require injury to the pleura to precede pathogen instillation [15–19], as direct bacterial administration into the pleural space often results in their complete clearance  or severe sepsis . Other studies have suggested that a pleural effusion is required as a substrate for bacterial growth [22, 23]. One commonly used method involves the intrapleural injection of turpentine, which results in pleural inflammation and an exudative effusion, prior to the introduction of pathogens . This approach, however, has variable efficacy  and turpentine itself may influence the results. Intrapleural injection of Pasturella multocida (a common respiratory tract pathogen in rabbits) in brain-heart infusion agar into rabbits has been shown to reliably induce empyema ; however, death from overwhelming sepsis frequently complicates this model unless systemic antibiotics are administered.
The technical issues associated with the current animal models of empyema preclude their use in large scale in vivo research. The aforementioned models differ from common clinical scenarios in that empyema is induced in the absence of concurrent pneumonia. The use of animal-specific pathogens and the need to administer antibiotics also limit the translational relevance of these models.
Effect of bacteria on mesothelial cells in vitro
In the absence of faithful animal models, most research on infection of serosal (pleural and peritoneal) cavities have been in vitro based. Numerous studies have described the potent biological effects of bacteria, live or attenuated, on both pleural and peritoneal mesothelial cells. The mesothelial cell responses appear to be dependent on both the state and type of microbial species and the origin of the mesothelial cells. Direct exposure of mesothelial cells to different types of bacteria have been shown to affect inflammatory responses, coagulation and fibrinolysis processes, resulting in a unique mesothelial cell activation signature . For example, the presence of heat-killed Escherichia coli increases elaboration of inflammatory cytokines such as interleukin (IL)-6, IL-8, RANTES (regulated upon activation, normal T-cell expressed and secreted) and monocyte chemotactic protein-1 (MCP-1) from mesothelial cells, whereas heat-killed Staphylococcus aureus increases IL-6 and IL-8 but not RANTES or MCP-1 . Variable activation was also observed in a separate study comparing the IL-8 response of peritoneal mesothelial cells cultured with live or heat-killed S. aureus, Staphylococcus epidermidis and E. coli or bacterial culture supernatants . The live S. aureus and live S. epidermidis isolates induced mesothelial cell production of IL-8, but the live E. coli or any of the heat-killed or bacterial culture supernatants did not, suggesting that heat alteration of the bacterial cell wall component may play a role . Peritoneal mesothelial cells have also been shown to ingest and kill live isolates of S. aureus, S. epidermidis and E. coli [27, 28]. While ingestion of Staphylococci was low, E. coli were digested within 8 h, resulting in mesothelial cell disintegration and bacterial proliferation .
Development of a parapneumonic pleural effusion and fibrinogenesis
Lung parenchymal infection stimulates local pleural immune activation, neutrophil migration and release of pro-inflammatory cytokines, such as IL-6, IL-8 and tumour necrosis factor (TNF)-α [29–31]. These mediators induce changes in the permeability of the mesothelial cell monolayer, which contributes to fluid accumulation in the pleural space. Murine studies of infection with S. aureus suggest pleural mesothelial cells express early response genes c-fos and c-jun, followed by the expression of pro-apoptotic genes Bak and Bad during late stage infection . This leads to pleural mesothelial cell apoptosis and loss of monolayer integrity, which may contribute to loss of the normal fibrinolytic milieu of the pleural space with conversion to a more pro-fibrinogenic, anti-fibrinolytic environment, with release of TNF-α from mesothelial cells  and concurrent upregulation of antifibrinolytic mediators, such as plasminogen activator inhibitor-1 and -2 . Infection of mesothelial cells with live mycobacteria bacille Calmette–Guerin (BCG) results in enhanced vascular endothelial growth factor release  and both live BCG and S. aureus infection increase permeability across the mesothelial monolayer, in part via downregulation of β-catenin [34, 35]. With persistent inflammation, increased vascular and mesothelial permeability leads to increased plasma extravasation into the pleural cavity. Activation of the coagulation cascade within the pleural cavity contributes to the development of a “fibrinopurulent” or “complicated” parapneumonic effusion, with fibrin deposition over both pleural surfaces and characteristic fibrinous septae producing loculated effusions .
The prevalence of different causative organisms of pleural infection varies among countries; local epidemiological data are needed to guide treatment. Series from the UK, Canada and New Zealand all demonstrate that Streptococcus milleri is the most common isolate in adults with community-acquired empyema, with proportions ranging between 32 and 50% of cases [4, 37, 38]. The other common isolated organisms of empyema are Streptococcus pneumoniae and anaerobes for community-acquired pleural infection and S. aureus (including methicillin-resistant S. aureus (MRSA)) for hospital-acquired cases . A more recent report from Taiwan has confirmed a higher incidence of aerobic Gram-negative and anaerobic infections in those with empyema from hospital-acquired pneumonia .
S. milleri is part of the viridans streptococci group that comprises a heterogeneous group of facultatively anaerobic, Gram-positive cocci that do not produce catalase or coagulase . S. milleri is a typically benign commensal that is part of the normal flora of the human orophayrnx, and it is rarely isolated in community-acquired pneumonia [40, 41], which makes its apparent virulence in empyema seem incongruous. Some reports suggest patients with S. milleri empyema more commonly have comorbidities, such as underlying malignancy or diabetes mellitus [42, 43], and mortality with S. milleri empyema has been reported to be up to one fifth of cases . A retrospective study of 72 patients in Taiwan identified that in 63% of cases anaerobes were co-isolated with S. milleri . It has been demonstrated in murine models of pulmonary infection that there may be synergy between S. milleri and anaerobes with greater histopathological abnormalities of pneumonia and higher mortality in the mixed infection group [44, 45]. Furthermore, viable bacteria were more numerous in the lungs of mice with mixed infections than in the lungs of those with single microbial infection. Accompanying in vitro studies suggested anaerobes may enhance the growth of the S. milleri group and/or inhibit the bactericidal activity of the host. Further research is required to better elucidate and understand the mechanisms by which this common bacteria causes pleural infection in humans.
The yield of bacterial culture of pleural fluid in empyema is low (∼60%) with conventional laboratory methods [37, 46]. Different postulations have been made to explain this observation. It may simply represent effective antibiotic treatment prior to sample collection. Alternatively, it may suggest that continual presence of bacteria is not necessary to sustain the ongoing inflammatory response after the initial bacterial invasion. Lack of sensitivity of conventional cultural techniques, presence of biofilms in the pleura and other hypotheses are also being examined.
In paediatric empyema series, S. pneumoniae is the most common organism and can account for up to 51% of cases [47, 48]. Interestingly, the incidence of paediatric empyema continues to rise despite the introduction of the pneumococcal vaccine, raising intriguing questions.
The heptavalent pneumococcal conjugate vaccine (PCV-7) for children, released in the late 1990s, targets pneumococcal strains 4, 6B, 9V, 14, 18C, 19F and 23F , which are traditionally associated with invasive pneumococcal disease, including empyema . PCV-7 reduces nasopharyngeal carriage [51–53] and the likelihood of community spread , resulting in a decrease in invasive pneumococcal disease in children , but also a reduction in adult invasive disease of the strains covered by the PCV-7 [55, 56]. The adult benefit from the PCV-7 programme in children continued to be observed for several years after its introduction in the USA .
It is, therefore, puzzling why many countries, including the USA [9, Spain 10], Israel  and Belgium , have reported a marked increase in the incidence of empyema in children with pneumonia. A similar trend to increasing incidence of empyema since 2000 has also been observed in adults , with a three- to five-fold increase in empyema incidence reported by groups in the USA  and Spain . As post-pneumonic empyema is much more common in children than adults [59, 60], it is logical that a change in incidence would be detected more quickly in this age group.
Determining the extent to which PCV-7 is responsible for the observed increase in empyema is problematic. While pneumococci are the most commonly identified cause of empyema in children and adults, S. aureus, including methicillin-resistant strains, are being reported with increased frequency in both adults [58 and children 61]. In some centres there was also clearly an increasing rate of empyema before the introduction of PCV-7 [59–61], suggesting that there may have been a shift in virulence of empyema-causing pneumococcal strains prior to vaccine introduction. However, in the reports of increasing empyema incidence after PCV-7, the non-vaccine serotype 1 has usually been the most commonly identified pneumococcus [58, 59, 62, 63].
Overall, it appears that there may have been a change in the invasive properties of empyema-causing pneumococcal serotypes prior to the introduction of PCV-7. Even without a change in virulence, the historically empyema-prone serotypes, predominantly 1 and 3, are not covered by PCV-7, so the widespread adoption of the vaccine has favoured their spread by reducing their natural competition. The 10-, 11-, and 13-valent PCV in development will cover the new non-PCV-7 strains that have become dominant. It will be intriguing to observe how this may alter the incidence of empyema and what new pathogens may fill the niche vacated.
The clinical manifestations of parapneumonic effusion vary according to the underlying infection; in the UK-based Multicentre Intrapleural Sepsis Trial (MIST) of 430 patients, the median (interquartile range) duration of symptoms prior to recruitment was 14 (8–28) days . Aerobic pneumonic infections will tend to present with an acute febrile illness, localised pleuritic chest pain, sputum production and leukocytosis. Infections with anaerobes tend to lead a more insidious clinical course with, less pronounced fever and more generalised systemic symptoms, such as poor appetite and weight loss; such infections are more common in those with poor dental hygiene, in alcoholics and in those who have had a period of unconciousness leading to aspiration of gastric contents .
Exudative simple parapneumonic effusions can occur in as many as 40% of patients with pneumonia , and may frequently resolve with appropriate antibiotic treatment of the pneumonia, without the need for drainage. The diagnosis of pleural infection is often delayed  and a high clinical suspicion is required to make an early and accurate diagnosis of a parapneumonic effusion. Pleural infection should be suspected in all patients with pneumonia, in particular those who fail to respond to appropriate antibiotic therapy, as defined by persistent fever, leukocytosis and raised inflammatory markers such as C-reactive protein. Immunosuppressed hosts and the elderly can present with disproportionately mild symptoms (relative to the severity of the pleural infection). The size of the effusion varies, and cannot be used to predict infective aetiology.
It is important to separate pleural infections that follow community-acquired pneumonia and hospital-acquired cases, as the causative bacteria are often different. Hospital-acquired empyema has a significantly worse outcome, with a mortality of up to 50% , and must be treated urgently and aggressively.
INVESTIGATION AND DIAGNOSIS
Any patient presenting with pneumonic symptoms, or those who are failing to respond to appropriate therapy and/or who have a pleural-based opacity on chest radiograph that obscures the hemidiaphragm, should be considered for further imaging and investigation. A lateral chest radiograph may be useful in this instance, where the presence of pleural fluid is suggested when the posterior costophrenic angle is blunted, or the hemidiaphragm is not visible throughout its length .
Ultrasound of the thoracic cavity (fig. 1) can reliably demonstrate loculations and septations , and increases the success rate and reduces the complications of thoracentesis . A recent retrospective study of 141 patients has demonstrated the appearance of sonographic septation to be a useful sign to help predict the outcome of small-bore catheter drainage in cases of empyema or complicated parapneumonic pleural effusion . Patients with a complex septated sonographic pattern had a poorer prognosis for a successful outcome, higher ICU admission rate and a higher mortality rate.
Contrast-enhanced computed tomography (CT) of the chest (fig. 2) will provide detailed information on parenchymal infiltrate, the presence of pulmonary abscess and position of chest drains, although a study of 50 patients suggested that CT abnormalities do not predict the stage of the parapneumonic effusion, nor its likelihood of requiring surgical intervention . Furthermore, radiological features on CT are not predictive of which patients require conversion to thoracotomy decortications from video-assisted thoracoscopic surgery (VATS), should surgery be required . A recent study of paediatric empyema in 60 patients treated with percutaneous chest drains and urokinase examined the role of routine CT in this population and found that, while CT did demonstrate more parenchymal abnormalities, its use did not alter management over and above ultrasound and chest radiograph ; as a result, the prevailing opinion suggests that in the paediatric population ultrasound should be routinely used, and CT only in special circumstances . CT should be used when the diagnosis is in doubt and may also provide information on underlying abnormalities associated with the empyema, such as pulmonary abscesses, an oesophageal perforation or bronchogenic carcinoma. Rarely, depending on the clinical setting, additional investigations may be required, such as a barium swallow (fig. 3).
Biochemical sampling of the pleural fluid is essential. Neutrophil phagocytic activity, with protease production and cell wall lysis, leads to a fall in pH of the pleural fluid  and concurrent fall in glucose, both characteristic biochemical features of complicated parapneumonic effusions and empyema [6, 76]. The increasing numbers of inflammatory cells within the pleural space lead to a rise in lactate dehydrogenase , often in excess of three times the upper limit of normal. A recent report has demonstrated that pleural fluid IL-8 can be an accurate discriminator of complicated and uncomplicated parapneumonic effusion , although the same report also demonstrated that pleural fluid pH was equivalent to IL-8 in such discrimination. As a result, pH remains the most useful in defining the need for pleural drainage [14, 79]. Accurate measurement of pleural fluid pH requires the use of blood gas analysers and careful collection without admixture of air or carried over lignocaine . Pleural fluid glucose correlates with pH and can be an alternative if accurate pH analysis is not available.
Many other biomarkers have been investigated for the diagnosis of pleural infection from simple parapneumonic effusions or effusions of other aetiologies, e.g. procalcitonin and TREM-1 (triggering receptor expressed on myeloid cells 1) [81, 82]. None of these markers have the accuracy to be used as a sole diagnostic tool. Also, they cannot replace conventional culture tests, as identification of the organism and antibiotic sensitivity are important to management.
Empyema is confirmed by the presence of frank pus, or positive microbial staining and/or culture of the pleural fluid. Diagnostic yield from culture will be increased with the innoculation of blood culture bottles with pleural fluid at the bedside  and the biochemical characteristics of the fluid in different locules may vary . Bacterial PCR techniques have been applied to pleural fluids with promising results . Recent evidence suggests that the use of a broad range 16S rDNA PCR may increase the chance of identifying pathogens (especially Gram-positive organisms) from pleural fluid [47, 48]. The use of PCR in adult empyema samples (with a wider range of organisms) has however, yielded mixed results, with significant false positives and negatives precluding its clinical use to date.
“If an empyema does not rupture, death will occur” – Hippocrates
The principal aims are to control the infection and to evacuate the infected material. Once pleural infection is suspected, appropriate broad spectrum systemic antibiotics must be started without delay. Choice of antibiotics depends on the local bacteriology and pathogen resistance patterns. The British Thoracic Society guidelines recommend a combination of cefuroxime and metronidazole or co-amoxiclav for community-acquired empyema in adults and vancomycin plus meropenem for hospital-acquired cases (because of the high incidence of MRSA) [46, 85]. Our knowledge is very limited regarding the pharmacokinetics of antibiotic activity in the pleura following intravenous or oral delivery: a topic for urgent research, which is needed to guide choice of therapy.
Drainage is most commonly achieved using an intercostal catheter. As many patients with pleural infection have multi-septated pleural collections, imaging-guided chest tube placement often allows optimal placement of the drainage tube in the largest collections of infected material, and are preferred over “blind” bedside insertion of chest tubes. Although large-bore chest tubes were often advocated for pus drainage, several observational series suggested that small-bore catheters can be adequate [71, 86, 87]. In case of multiple large loculations, more than one catheter may be needed to achieve adequate pus evacuation. In the MIST study, no significant differences were found in the outcomes of patients who received large chest drains and those treated with small-bore catheters , although the assignment of catheters was not randomised. Blockage is more common with small-bore drains  and regular flushing of the drains may be necessary . Small-bore catheter sets frequently contain an integral three-way tap, the internal diameter of which is often ≤9 French gauge and thus act as the rate-limiting point for drainage.
The use of indwelling small-bore pleural catheters for ambulatory drainage of chronic empyema has been shown to be successful in anecdotal cases . Repeated (imaging-guided) thoracentesis can theoretically be an alternative to chest tube drainage and has been shown to be effective in animal experiments as well as in one small clinical study . The concept of lavaging the pleural space has also been explored  and some European centres advocate intrapleural infusion of saline several times a day to “wash out” the pleural space to reduce bacterial load. These approaches would require further evaluation.
Septations often prohibit complete evacuation of the pleural pus and the presence of septated empyema has been demonstrated to prolong hospital stay and chest tube drainage time, and to increase the need for surgery  and predict failure of tube drainage . Fibrinolytics have been utilised in an attempt to lyse loculations in order to enhance drainage, and while initial studies have shown that intrapleural administration of streptokinase or urokinase significantly increases drainage volume, fibrinolytics do not improve patient outcome . Animal studies have suggested that streptokinase can provoke fluid accumulation even in a normal pleural cavity, and may explain the dramatic (up to nine-fold) increase in pleural fluid drainage following fibrinolytic therapy [87, 96].
The largest study to date, a randomised double-blind trial of 430 patients showed that intrapleural streptokinase did not reduce mortality or the need for salvage surgical drainage , with similar findings echoed in a single-centre study from South Africa . Use of other fibrinolytics, such as tissue plasminogen activator, either alone or combined with DNase (which reduces pus viscosity in vitro and in isolated case reports), have shown benefits in one small study of 20 adults , and have been assessed in a large UK randomised clinical trial, which is due to be reported shortly.
Surgery for empyema
Surgery should be considered without delay in patients who fail to improve with antibiotics and chest tube drainage, and who have persistent infective symptoms, fever, leukocytosis and raised inflammatory markers. Contrary to popular belief, radiological clearance of pleural collection is not a good indicator of disease progress. Two recent longitudinal studies have shown that radiological opacity of pleural infection improves in both adult and paediatric patients over the subsequent months, without the need for surgery [4, 97]. Likewise, restrictive changes in pulmonary function tests usually improve in parallel over time; very few patients have functional impairment from residual pleural fibrosis.
In several retrospective studies, those referred late for surgery had more complications and lower success rate from VATS, and longer hospital stay . VATS is the procedure of choice, as it is equally effective but less invasive (and hence results in shorter hospital stay and fewer complications), than drainage by thoracotomy in both adults and children [99–101], although in up to 20% of patients, VATS is inadequate and conversion to open thoracotomy drainage is necessary . Medical thoracoscopy has been shown to be safe and effective in one retrospective series of 127 patients . A variety of surgical techniques have been utilised and a detailed description can be found elsewhere .
The role of “primary surgical treatment” as the first-line approach for empyema is unclear. To date, two randomised trials have compared primary VATS against conservative chest tube drainage in adult patients and neither showed any major clinical benefits, except for a slightly shorter hospital stay (mean±sd; 8.7±0.9 versus 12.8±1.1 days) [105, 106]. Two randomised trials in paediatric patients also failed to show significant advantages of surgery [102, 107], although both of these studies suggested lower treatment costs in the fibrinolysis groups, although these were secondary analyses. No significant cost differentials exist between primary VATS and conservative chest tube drainage in any formal published health economy analyses.
Treatment of underlying clinical predisposing factors, such as poor dentition or immunodeficiency, is essential, and nutritional support is important , with a recent large analysis of prognostic factors in community-acquired empyema identifying an admission serum albumin of ≤30 g·L−1 as an independent risk factor for mortality .
Publication of this peer-reviewed article was supported by GlaxoSmithKline, Belgium (principal sponsor, European Respiratory Review issue 117).
Statement of Interest
Y.C.G. Lee is a co-investigator of the British Thoracic Society Multicentre Intrapleural Sepsis Trial 2, which is partly funded by Roche Ltd.
- Received May 25, 2010.
- Accepted May 27, 2010.
- ©ERS 2010